Expression of the multidrug resistance gene in human tumors.
Expression of the multidrug resistance gene in human tumors.
复制标题
多药耐药基因在人类肿瘤中的表达。
DOI：
10.11501/3052469
复制
发表时间：
2014
影响因子：
--
通讯作者：
Rung
中科院分区：
文献类型：
--
作者：
Rung
研究了MDR 1基因在人实体瘤中的表达与阿霉素耐药的关系。40例新鲜人手术标本通过RNA斑点印迹法分析其人MDR1基因的表达，并使用抗P-糖蛋白（MDR1基因产物）的单克隆抗体进行免疫组织化学染色。40例肿瘤患者中有11例MDR1 mRNA表达增高，其中直肠癌3例，乳腺癌2例，胃癌2例，结肠癌1例，肾癌1例，胆囊癌1例，胃恶性淋巴瘤1例。然而，在特定类型的癌症中注意到MDR1 mRNA水平的相当大的变化。免疫组化研究表明，与单克隆抗体在18个肿瘤呈阳性。在所有检测的肿瘤中，MDR1 mRNA水平和免疫组化分析显示出显著的相关性。5例耐药肿瘤中2例MDR1表达阴性，免疫组化染色阳性。这些结果表明，免疫组化分析将是更敏感的检测P-糖蛋白的表达，耐阿霉素，是多因素的，可以至少，部分与MDR 1基因产物的量增加。
The expression of MDR1 gene was investigated in human solid tumors with respect to adriamycin resistance. Forty fresh human surgical specimens were analyzed by RNA dot blot assay for their expression of the human MDR1 gene and by immunohistological staining using a monoclonal antibody against P-glycoprotein (MDR1 gene product). The MDR1 mRNA level was increased in 11 cases of 40 cancer patients, including three rectal cancers, two breast cancers, two gastric cancers, one colon cancer, one renal cell carcinoma, one gall bladder cancer and one malignant lymphoma of stomach. However, considerable variation of the MDR1 mRNA level was noted among cancers of a specific type. Immunohistochemical studies with the monoclonal antibody were shown to be positive in 18 tumors. In all tumors tested, the MDR1 mRNA level and the immunohistochemical analysis showed a significant correlation. However, two of five tumors which resisted adriamycin treatment were found to be negative in MDR1 transcript, but positive in immunohistological analysis. These results indicate that immunohistochemical analysis would be more sensitive for detecting P-glycoprotein-expression, and that resistance to adriamycin, being multifactorial, can be associated at least, in part with the increased amount of MDR1 gene product.
影响因子：
2.6
作者：
Mahmoudi S;Yaghmaei B;Sharifzadeh Ekbatani M;Pourakbari B;Navaeian A;Parvaneh N;Haghi Ashtiani MT;Mamishi S
通讯作者：
Mamishi S