Effects of Coronavirus Disease 2019 (COVID-19) on Peripheral Blood Lymphocytes and Their Subsets in Children: Imbalanced CD4(+)/CD8(+) T Cell Ratio and Disease Severity.
Effects of Coronavirus Disease 2019 (COVID-19) on Peripheral Blood Lymphocytes and Their Subsets in Children: Imbalanced CD4(+)/CD8(+) T Cell Ratio and Disease Severity.
复制标题
DOI：
10.3389/fped.2021.643299
复制
发表时间：
2021
影响因子：
2.6
通讯作者：
Mamishi S
中科院分区：
文献类型：
--
作者：
Mahmoudi S;Yaghmaei B;Sharifzadeh Ekbatani M;Pourakbari B;Navaeian A;Parvaneh N;Haghi Ashtiani MT;Mamishi S
简介：虽然COVID-19的发病机制尚不完全清楚，SARS-CoV-2与免疫系统之间的影响也很复杂，但已知淋巴细胞减少症、过度炎症反应和细胞因子在COVID-19的病理学中起着重要作用。虽然在COVID-19的实验室特征中描述了一些血液学异常，但尚未有关于儿童淋巴细胞亚群分析的研究报告。本研究的目的是描述轻度/中度或重度COVID-19儿科患者的淋巴细胞亚群。研究方法：研究对象为经实时荧光定量RT-PCR确诊的严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）肺炎患儿。受试者于2020年3月7日至6月10日入住德黑兰医科大学附属儿童医学中心。分析每例患者的全血细胞计数和淋巴细胞亚群。结果如下：该研究包括55名确诊为SARS-CoV-2感染的住院患者（34名患者（62%）观察到轻度/中度病例，21名患者（38%）患有严重疾病）。发现重度病例患者的淋巴细胞计数较低（重度组的平均值± SD为1.6 ± 0.9，轻度组为2.3 ± 2.2）。与轻、中度肺炎组相比，重症肺炎组患儿CD 8 + T细胞计数升高，CD 4 + T细胞百分比降低。然而，两组之间的差异可以忽略不计。有趣的是，与轻度组相比，重度组具有较低的CD 4 +/CD 8 + T细胞比率（1.1 ± 0.47 vs. 1.4 ± 0.8，p值：0.063）。CD 4 +/CD 8 + T细胞比值<2者48例（87%），<1.5者40例（73%），<1者19例（35%）。受试者通过的所有7个病例（13%）的CD 4 +/CD 8 + T细胞比率<2，86%的CD 4 +/CD 8 + T细胞比率<1.5，29%的CD 4 +/CD 8 + T细胞比率<1。结论：重度COVID-19患者的CD 4 +/CD 8 + T细胞比率低于轻/中度疾病患者。然而，尽管CD 4 +/CD 8+比值的下降可能是分析严重COVID-19患者免疫反应紊乱的有用指标，但强烈建议进一步研究更大的样本量。
Introduction: While pathogenesis in COVID-19 is not fully known and the effects between SARS-CoV-2 and the immune system are complicated, it is known that lymphopenia, hyper-inflammatory responses, and cytokines play an important role in the pathology of COVID-19. While some hematological abnormalities have been described among the laboratory features of COVID-19, there have not been studies reported on lymphocyte subset analyses in children. The aim of this study was to describe lymphocyte subsets in pediatric patients with mild/moderate or severe COVID-19. Methods: The subjects in the study were children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia confirmed with the real-time RT-PCR. The subjects were admitted to the Children's Medical Center, affiliated with the Tehran University of Medical Sciences, between March 7th and June 10th of 2020. The complete blood counts and lymphocyte subpopulations were analyzed for each patient. Results: The study included 55 hospitalized patients with confirmed SARS-CoV-2 infection (34 patients (62%) with an observed mild/moderate case of the disease and 21 patients (38%) with severedisease). Lymphocyte counts were found to be lower in patients with a severe case (mean ± SD 1.6 ± 0.9 in the severe group vs. 2.3 ± 2.2 in the mild group). Compared to the group with mild/moderate pneumonia, children with severe pneumonia had an increased count of CD8+ T cell and a lower percentage of CD4+ T cell. However, the differences between the groups were negligible. Interestingly, the severe group had a lower CD4+/CD8+ T cell ratio compared to the mild group (1.1 ± 0.47 vs. 1.4 ± 0.8, p-value: 0.063). CD4+/CD8+ T cell ratio <2, 1.5, and 1 was found in 48 (87%), 40 (73%), and 19 cases (35%). All of the seven cases in which the subject passed (13%) had CD4+/CD8+ T cell ratio of <2, 86% had CD4+/CD8+ T cell ratio of <1.5, and 29% had CD4+/CD8+ T cell ratio of <1. Conclusion: The CD4+/CD8+ T cell ratio was lower in patients with severe COVID-19 compared to those with mild/moderate form of disease. However, although a decline in CD4+/CD8+ ratio may serve as a useful metric in analyzing of the derangement in immune responses in patients with severe COVID-19, further study with larger sample sizes is highly recommended.
登录
查看更多内容
DOI：
10.1016/s0140-6736(03)13364-8
发表时间：
2003-05-17
期刊：
Lancet (London, England)
影响因子：
--
作者：
Hon KL;Leung CW;Cheng WT;Chan PK;Chu WC;Kwan YW;Li AM;Fong NC;Ng PC;Chiu MC;Li CK;Tam JS;Fok TF
通讯作者：
Fok TF
影响因子：
3.9
作者：
Mahmoudi, Shima;Mehdizadeh, Mehrzad;Mamishi, Setareh
通讯作者：
Mamishi, Setareh
影响因子：
82.9
作者：
MARGOLICK, JB;MUNOZ, A;FERBAS, J
通讯作者：
FERBAS, J
影响因子：
4.4
作者：
Hadrup, SR;Strindhall, J;Wikby, A
通讯作者：
Wikby, A
影响因子：
8
作者：
Bitnun, A;Allen, U;Read, S
通讯作者：
Read, S