The transcriptional repressor ZBP-89 and the lack of Sp1/Sp3, c-Jun and Stat3 are important for the down-regulation of the vimentin gene during C2C12 myogenesis.
The transcriptional repressor ZBP-89 and the lack of Sp1/Sp3, c-Jun and Stat3 are important for the down-regulation of the vimentin gene during C2C12 myogenesis.
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DOI：
10.1016/j.diff.2008.12.005
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发表时间：
2009-06
期刊：
影响因子：
2.9
通讯作者：
Zehner, Zendra E.
中科院分区：
文献类型：
--
作者：
Salmon, Morgan;Zehner, Zendra E.
Currently, considerable information is available about how muscle-specific genes are activated during myogenesis, yet little is known about how non-muscle genes are down-regulated. The intermediate filament protein vimentin is known to be “turned off” during myogenesis to be replaced by desmin, the muscle-specific intermediate filament protein. Here, we demonstrate that vimentin down-regulation is the result of the combined effect of several transcription factors. Levels of the positive activators, Sp1/Sp3, which are essential for vimentin expression, decrease during myogenesis. In addition, c-Jun and Stat3, two additional positive-acting transcription factors for vimentin gene expression, are also down-regulated. Over-expression via adenoviral approaches demonstrates that the up-regulation of the repressor ZBP-89 is critical to vimentin down-regulation. Elimination of ZBP-89 via siRNA blocks the down-regulation of vimentin and Sp1/Sp3 expression. From these studies we conclude that the combinatorial effect of the down-regulation of positive-acting transcription factors such as Sp1/Sp3, c-Jun and Stat3 versus the up-regulation of the repressor ZBP-89 contributes to the “turning off” of the vimentin gene during myogenesis.
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影响因子：
3.5
作者：
Izmailova, ES;Zehner, ZE
通讯作者：
Zehner, ZE
DOI：
10.1006/bbrc.1999.1180
发表时间：
1999-08-19
影响因子：
3.1
作者：
Law, DJ;Du, M;Merchant, JL
通讯作者：
Merchant, JL
影响因子：
7.5
作者：
PARRY D A D;STEINERT P M
通讯作者：
STEINERT P M
影响因子：
4.8
作者：
Andreucci, JJ;Grant, D;McDermott, JC
通讯作者：
McDermott, JC
影响因子：
4.8
作者：
Chupreta, Sergey;Brevig, Holly;Iniguez-Lluhi, Jorge A.
通讯作者：
Iniguez-Lluhi, Jorge A.