Comparative genomic characterization of a Thailand-Myanmar isolate, MS6, of Vibrio cholerae O1 El Tor, which is phylogenetically related to a "US Gulf Coast" clone.
Comparative genomic characterization of a Thailand-Myanmar isolate, MS6, of Vibrio cholerae O1 El Tor, which is phylogenetically related to a "US Gulf Coast" clone.
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DOI：
10.1371/journal.pone.0098120
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hamada S
中科院分区：
文献类型：
--
作者：
Okada K;Na-Ubol M;Natakuathung W;Roobthaisong A;Maruyama F;Nakagawa I;Chantaroj S;Hamada S
2007-2010年期间泰国的霍乱暴发完全是由携带经典生物型霍乱毒素基因的霍乱弧菌O 1 El Tor变种引起的。我们先前从腹泻患者中分离出霍乱弧菌O 1 El Tor菌株，并将其命名为MS 6。多位点序列分型分析表明，MS 6与U. S.除了两个新的管家基因外，墨西哥湾沿岸的克隆。测定了MS 6的基因组核苷酸序列，并与世界各地临床和环境中分离的26株霍乱弧菌进行了比较。我们在这里表明，MS 6分离株与正在进行的第七次大流行霍乱弧菌O 1 El Tor菌株有远亲关系。这些菌株的不同之处在于管家基因、第七大流行群特异性标志物、CTX β、编码预测的跨膜蛋白的两个基因、霍乱弧菌O 1血清群高度保守区域中metY（MS6_A0927）或hchA/luxR的存在以及超级整合子（SI）的多态性。我们发现霍乱弧菌携带hchA/luxR或metY，霍乱弧菌O 1分支通常具有hchA/luxR，除了MS 6和U。S.墨西哥湾沿岸菌株。这些发现阐明了霍乱弧菌O 1菌株之间的进化关系。此外，MS 6 SI携带喹诺酮类耐药基因盒，这是密切相关的质粒携带的整合子的其他革兰阴性菌。系统发育分析表明，MS 6与美国的一个种关系最为密切。S.墨西哥湾沿岸的克隆，表明他们的分歧之前，埃尔托生物型菌株从一个共同的霍乱弧菌O 1祖先。我们建议，MS 6作为一个环境的水生水库V。霍乱O 1。
The cholera outbreaks in Thailand during 2007–2010 were exclusively caused by the Vibrio cholerae O1 El Tor variant carrying the cholera toxin gene of the classical biotype. We previously isolated a V. cholerae O1 El Tor strain from a patient with diarrhea and designated it MS6. Multilocus sequence-typing analysis revealed that MS6 is most closely related to the U. S. Gulf Coast clone with the exception of two novel housekeeping genes. The nucleotide sequence of the genome of MS6 was determined and compared with those of 26 V. cholerae strains isolated from clinical and environmental sources worldwide. We show here that the MS6 isolate is distantly related to the ongoing seventh pandemic V. cholerae O1 El Tor strains. These strains differ with respect to polymorphisms in housekeeping genes, seventh pandemic group-specific markers, CTX phages, two genes encoding predicted transmembrane proteins, the presence of metY (MS6_A0927) or hchA/luxR in a highly conserved region of the V. cholerae O1 serogroup, and a superintegron (SI). We found that V. cholerae species carry either hchA/luxR or metY and that the V. cholerae O1 clade commonly possesses hchA/luxR, except for MS6 and U. S. Gulf Coast strains. These findings illuminate the evolutionary relationships among V. cholerae O1 strains. Moreover, the MS6 SI carries a quinolone-resistance gene cassette, which was closely related with those present in plasmid-borne integrons of other gram-negative bacteria. Phylogenetic analysis reveals that MS6 is most closely related to a U. S. Gulf Coast clone, indicating their divergence before that of the El Tor biotype strains from a common V. cholerae O1 ancestor. We propose that MS6 serves as an environmental aquatic reservoir of V. cholerae O1.
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影响因子：
14.9
作者：
Hunter S;Jones P;Mitchell A;Apweiler R;Attwood TK;Bateman A;Bernard T;Binns D;Bork P;Burge S;de Castro E;Coggill P;Corbett M;Das U;Daugherty L;Duquenne L;Finn RD;Fraser M;Gough J;Haft D;Hulo N;Kahn D;Kelly E;Letunic I;Lonsdale D;Lopez R;Madera M;Maslen J;McAnulla C;McDowall J;McMenamin C;Mi H;Mutowo-Muellenet P;Mulder N;Natale D;Orengo C;Pesseat S;Punta M;Quinn AF;Rivoire C;Sangrador-Vegas A;Selengut JD;Sigrist CJ;Scheremetjew M;Tate J;Thimmajanarthanan M;Thomas PD;Wu CH;Yeats C;Yong SY
通讯作者：
Yong SY
影响因子：
9.4
作者：
Nair, G. Balakrish;Qadri, Firdausi;Sack, David A.
通讯作者：
Sack, David A.
影响因子：
9.4
作者：
Nandi, B;Nandy, RK;Ghose, AC
通讯作者：
Ghose, AC
DOI：
10.1073/pnas.95.6.3134
发表时间：
1998-03-17
影响因子：
11.1
作者：
Karaolis, DKR;Johnson, JA;Reeves, PR
通讯作者：
Reeves, PR
影响因子：
9.4
作者：
O'Shea, YA;Reen, FJ;Boyd, EF
通讯作者：
Boyd, EF