Pathologic changes and contractile properties of the diaphragm in corticosteroid myopathy in hamsters: comparison to peripheral muscle.
Pathologic changes and contractile properties of the diaphragm in corticosteroid myopathy in hamsters: comparison to peripheral muscle.
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仓鼠皮质类固醇肌病膈肌的病理变化和收缩特性：与周围肌肉的比较。
DOI：
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发表时间：
1989
影响因子：
6.4
通讯作者：
R. Pardy
中科院分区：
文献类型：
--
作者：
Pearce G. Wilcox;Jennifer M. Hards;K. Bockhold;B. Bressler;R. Pardy
已显示皮质类固醇产生外周骨骼肌肌病，其主要特征为II型纤维萎缩。为了确定类似的组织学和组织化学变化是否发生在隔膜和是否在体外收缩性能的肌肉受到类固醇的不利影响，我们研究了两组仓鼠。实验组给予曲安奈德，对照组给予生理盐水，均每日肌肉注射，持续3周。注射剂比目鱼肌（Sol）和趾长伸肌（EDL）和肋膈肌切片进行染色，用于组织学（苏木精和伊红，改良Gomori三色法）和组织化学（肌球蛋白ATP酶，琥珀酸脱氢酶[SDH]）分析。肌纤维比例和横截面积（CSA）从肌球蛋白ATP酶切片测量。在离体研究中进行了等距收缩的小条肋膈，测量最大等长收缩（Pt）和强直（Po）的张力，时间达到峰值张力（TTP），半松弛时间（1/2 RT），力-频率关系，和疲劳特性（60赫兹强直;占空比，0.5）。曲安西龙治疗导致肌纤维比例没有变化。对I型纤维CSA无影响;然而，在曲安奈德给药动物中存在IIa型（Sol，EDL）和IIb型（膈肌，EDL）纤维萎缩。隔膜条的Pt和Po（标准化重量）无差异。经曲安奈德处理的膈肌的TTP和1/2 RT延长，力-频曲线左移，疲劳性降低（P <0.05）。我们的结论是，类固醇肌病可以解释为肌肉质量的损失（IIb型纤维萎缩），而不是在收缩功能的内在损害。（250字处删节）
Corticosteroids have been shown to produce a myopathy of peripheral skeletal muscle, characterized predominantly by Type II fiber atrophy. To determine if similar histologic and histochemical changes occur in the diaphragm and whether the in vitro contractile properties of this muscle are adversely affected by steroids, we studied two groups of hamsters. The experimental group received triamcinolone while a control group received saline, both given daily for 3 wk as i.m. injections. Soleus (Sol) and extensor digitorum longus (EDL) muscles and costal diaphragm muscle sections were stained for histologic (hematoxylin and eosin, modified Gomori trichrome) and histochemical (myosin ATPase, succinate dehydrogenase [SDH]) analysis. Muscle fiber proportions and cross-sectional areas (CSA) were measured from myosin ATPase sections. In vitro studies of isometric contractions were carried out on small strips of costal diaphragm, measuring maximal isometric twitch (Pt) and tetanus (Po) tensions, time to peak tension (TTP), half relaxation time (1/2 RT), force-frequency relationship, and fatigue characteristics (60 Hz tetani; duty cycle, 0.5). Triamcinolone treatment resulted in no change in muscle fiber proportions. There was no effect on Type I fiber CSA; however, there was Type IIa (Sol, EDL) and Type IIb (diaphragm, EDL) fiber atrophy in triamcinolone-treated animals. Pt and Po (normalized for weight) of diaphragm strips were not different. There was a prolongation in TTP and 1/2 RT, a left shift in the force-frequency curve, and a reduced fatiguability of triamcinolone-treated diaphragm (P less than 0.05). We conclude that a steroid myopathy could be explained by a loss of muscle mass (Type IIb fiber atrophy) rather than an intrinsic impairment in contractile function.(ABSTRACT TRUNCATED AT 250 WORDS)
DOI：
10.1152/jappl.1985.58.4.1354
发表时间：
1985
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Kelsen,SG;Ference,M;Kapoor,S
通讯作者：
Kapoor,S
雄激素胞质结合在运动引起的肌肉萎缩中的保留。
DOI：
10.1152/ajpendo.1984.247.5.e597
发表时间：
1984
期刊：
The American journal of physiology
影响因子：
--
作者：
Hickson,RC;Kurowski,TT;Capaccio,JA;ChattertonJr,RT
通讯作者：
ChattertonJr,RT
影响因子：
3.3
作者：
DREW, JS;FARKAS, GA;ROCHESTER, DF
通讯作者：
ROCHESTER, DF