Oceans of opportunity: exploring vertebrate hematopoiesis in zebrafish.
Oceans of opportunity: exploring vertebrate hematopoiesis in zebrafish.
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DOI：
10.1016/j.exphem.2014.05.002
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发表时间：
2014-08
影响因子：
2.6
通讯作者：
North, Trista E.
中科院分区：
文献类型：
--
作者：
Carroll, Kelli J.;North, Trista E.
近年来，斑马鱼模型在血液学研究中的应用激增，成为了解造血发育、动态平衡和恶性肿瘤调节的最有用和最容易处理的系统之一。尽管斑马鱼与人类的进化距离很远，但在造血系统中显著的遗传和表型保守使我们对血液干细胞和祖细胞(HSPC)生物学的理解有了显著的进步。斑马鱼在血液学研究中的优势在于能够在体内实时观察造血干细胞、祖细胞和效应细胞的出现、扩增和功能，以及易于识别和表征特定造血过程或细胞类型的新型遗传和化学修饰物。此外，已经开发了无数的转基因系，包括荧光报告系统，以帮助可视化和量化特定的感兴趣的细胞类型和细胞-谱系关系，以及可用于实施广泛的实验操作的效应系。随着我们对HSPC生物学在发育过程中、对感染或损伤的反应或在血液系统恶性肿瘤背景下的复杂性质的理解不断加深，斑马鱼仍将是探索这些基本过程的时空组织和整合以及识别有效的造血活性小分子修饰物的关键。在这篇综述中，我们讨论了斑马鱼造血系统的生物学，包括与哺乳动物的异同，并强调了目前在斑马鱼胚胎和成体中用于增强我们对脊椎动物血液学的了解的重要工具，重点是影响我们对人类血液病和疾病的发病或治疗的理解的发现。
Exploitation of the zebrafish model in hematology research has surged in recent years, becoming one of the most useful and tractable systems for understanding regulation of hematopoietic development, homeostasis, and malignancy. Despite the evolutionary distance between zebrafish and humans, remarkable genetic and phenotypic conservation in the hematopoietic system has enabled significant advancements in our understanding of blood stem and progenitor cell (HSPC) biology. The strengths of zebrafish in hematology research lie in the ability to perform real-time in vivo observations of hematopoietic stem, progenitor and effector cell emergence, expansion and function, as well as the ease with which novel genetic and chemical modifiers of specific hematopoietic processes or cell-types can be identified and characterized. Further, a myriad of transgenic lines have been developed including fluorescent reporter systems to aid in the visualization and quantification of specified cell types of interest and cell-lineage relationships, as well as effector lines that can be used to implement a wide range of experimental manipulations. As our understanding of the complex nature of HSPC biology during development, in response to infection or injury, or in the setting of hematological malignancy, continues to deepen, zebrafish will remain essential for exploring the spatio-temporal organization and integration of these fundamental processes, as well as the identification of efficacious small molecule modifiers of hematopoietic activity. In this review, we discuss the biology of the zebrafish hematopoietic system, including similarities and differences from mammals, and highlight important tools currently utilized in zebrafish embryos and adults to enhance our understanding of vertebrate hematology, with emphasis on findings that have impacted our understanding of the onset or treatment of human hematologic disorders and disease.
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影响因子：
64.5
作者：
Bai X;Kim J;Yang Z;Jurynec MJ;Akie TE;Lee J;LeBlanc J;Sessa A;Jiang H;DiBiase A;Zhou Y;Grunwald DJ;Lin S;Cantor AB;Orkin SH;Zon LI
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Zon LI
影响因子：
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Speck, Nancy A.
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6.5
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Lin S
影响因子：
30.8
作者：
Brownlie, A;Donovan, A;Zon, LI
通讯作者：
Zon, LI
影响因子：
14.9
作者：
Cade L;Reyon D;Hwang WY;Tsai SQ;Patel S;Khayter C;Joung JK;Sander JD;Peterson RT;Yeh JR
通讯作者：
Yeh JR