Sodium hydroxide based non-detergent decellularizing solution for rat lung.
Sodium hydroxide based non-detergent decellularizing solution for rat lung.
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DOI：
10.1080/15476278.2018.1462432
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发表时间：
2018
期刊：
影响因子：
2.3
通讯作者：
Nagayasu T
中科院分区：
文献类型：
--
作者：
Sengyoku H;Tsuchiya T;Obata T;Doi R;Hashimoto Y;Ishii M;Sakai H;Matsuo N;Taniguchi D;Suematsu T;Lawn M;Matsumoto K;Miyazaki T;Nagayasu T
肺移植是治疗终末期慢性肺部疾病的最后选择。由于供体肺器官的短缺是主要障碍，因此肺再生被认为可以克服这一障碍。脱细胞器官支架的再细胞化是用于器官再生的有希望的选择。尽管去污剂通常用于脱细胞化，但其他方法也是可能的。在此，我们使用不含去污剂的高碱性（pH 12）氢氧化钠（NaOH）-PBS溶液进行肺脱细胞，并比较了基于去污剂的脱细胞溶液CHAPS和SDS对DNA消除和ECM保存的功效。免疫组化图像分析显示，细胞成分被NaOH溶液以及其他去污剂去除。胶原和GAG测定显示NaOH组的胶原减少与CHAPS和SDS组相当。然而，NaOH组的DNA减少比其他组更显著（p < 0.0001）。HUVEC的再细胞化显示细胞粘附不劣于SDS组。离体功能分析显示，在SDS或NaOH组中，当人工血红蛋白囊泡-PBS溶液通过再生肺时，100%氧气通气增加了氧分压。得出的结论是，基于NaOH-PBS的脱细胞溶液与普通脱细胞溶液相当，并且在成本效益方面具有竞争力，并且脱细胞支架中的残留物可忽略不计，因此为未来的临床使用提供了另一种潜在的去污剂选择。
Lung transplantation is the last option for the treatment of end stage chronic lung disorders. Because the shortage of donor lung organs represents the main hurdle, lung regeneration has been considered to overcome this hurdle. Recellularization of decellularized organ scaffold is a promising option for organ regeneration. Although detergents are ordinarily used for decellularization, other approaches are possible. Here we used high alkaline (pH12) sodium hydroxide (NaOH)-PBS solution without detergents for lung decellularization and compared the efficacy on DNA elimination and ECM preservation with detergent based decellularization solutions CHAPS and SDS. Immunohistochemical image analysis showed that cell components were removed by NaOH solution as well as other detergents. A Collagen and GAG assay showed that the collagen reduction of the NaOH group was comparable to that of the CHAPS and SDS groups. However, DNA reduction was more significant in the NaOH group than in other groups (p < 0.0001). The recellularization of HUVEC revealed cell attachment was not inferior to that of the SDS group. Ex vivo functional analysis showed 100% oxygen ventilation increased oxygen partial pressure as artificial hemoglobin vesicle-PBS solution passed through regenerated lungs in the SDS or NaOH group. It was concluded that the NaOH-PBS based decellularization solution was comparable to ordinal decellularizaton solutions and competitive in cost effectiveness and residues in the decellularized scaffold negligible, thus providing another potential option to detergent for future clinical usage.
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DOI：
10.1126/science.1189345
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Petersen TH;Calle EA;Zhao L;Lee EJ;Gui L;Raredon MB;Gavrilov K;Yi T;Zhuang ZW;Breuer C;Herzog E;Niklason LE
通讯作者：
Niklason LE
影响因子：
3
作者：
Tsuchiya, Tomoshi;Balestrini, Jenna L.;Niklason, Laura E.
通讯作者：
Niklason, Laura E.
影响因子：
82.9
作者：
Ott, Harald C.;Matthiesen, Thomas S.;Taylor, Doris A.
通讯作者：
Taylor, Doris A.
影响因子：
15
作者：
EHRLICH, P;DOTY, P
通讯作者：
DOTY, P
DOI：
10.1165/rcmb.2012-0509oc
发表时间：
2013-11-01
影响因子：
6.4
作者：
Kligys, Kristina;Wu, Yvonne;Jones, Jonathan C. R.
通讯作者：
Jones, Jonathan C. R.