Parthenogenesis-derived multipotent stem cells adapted for tissue engineering applications.
Parthenogenesis-derived multipotent stem cells adapted for tissue engineering applications.
复制标题
孤雌生殖衍生的多能干细胞适用于组织工程应用。
DOI：
10.1016/j.ymeth.2008.08.002
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发表时间：
2009-02
期刊：
影响因子：
4.8
通讯作者：
Atala, Anthony
中科院分区：
文献类型：
--
作者：
Koh, Chester J.;Delo, Dawn M.;Lee, Jang Won;Siddiqui, M. Minhaj;Lanza, Robert P.;Soker, Shay;Yoo, James J.;Atala, Anthony
当供体组织不可用时，胚胎干细胞被设想为用于再生医学应用的多能细胞的可行来源。然而，目前大多数胚胎干细胞的收获技术需要破坏胚胎，这导致了对其使用的重大政治和伦理限制。孤雌生殖，即卵子在没有精子的情况下发育成胚胎的过程，可能是胚胎干细胞的潜在来源，可以避免围绕胚胎干细胞的一些政治和伦理问题。在这里，我们提供了从卵母细胞孤雌激活中分离和扩增胚胎干细胞的技术方面。这些细胞的特征在于它们的干细胞特性。此外，这些细胞被诱导分化成肌源性、成骨性、成脂性和内皮性谱系，并且能够在体内形成肌样和骨样组织。此外，孤雌生殖干细胞能够整合到受伤的肌肉组织中。总之，这些结果表明，孤雌生殖干细胞可以成功地分离和用于各种组织工程应用。
Embryonic stem cells are envisioned as a viable source of pluripotent cells for use in regenerative medicine applications when donor tissue is not available. However, most current harvest techniques for embryonic stem cells require the destruction of embryos, which has led to significant political and ethical limitations on their usage. Parthenogenesis, the process by which an egg can develop into an embryo in the absence of sperm, may be a potential source of embryonic stem cells that may avoid some of the political and ethical concerns surrounding embryonic stem cells. Here we provide the technical aspects of embryonic stem cell isolation and expansion from the parthenogenetic activation of oocytes. These cells were characterized for their stem-cell properties. In addition, these cells were induced to differentiate to the myogenic, osteogenic, adipogenic, and endothelial lineages, and were able to form muscle-like and bony-like tissue in vivo. Furthermore, parthenogenetic stem cells were able to integrate into injured muscle tissue. Together, these results demonstrate that parthenogenetic stem cells can be successfully isolated and utilized for various tissue engineering applications.
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影响因子：
64.8
作者：
Park, In-Hyun;Zhao, Rui;Daley, George Q.
通讯作者：
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影响因子：
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West, MD
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通讯作者：
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影响因子：
5.2
作者：
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