Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer.
Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer.
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DOI：
10.5114/wo.2012.28794
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Kaczmarek E
中科院分区：
文献类型：
--
作者：
Kasprzak A;Szaflarski W;Szmeja J;Andrzejewska M;Przybyszewska W;Koczorowska M;Drews M;Kaczmarek E
几项流行病学研究试图证明血清胰岛素样生长因子1（IGF-1）水平升高与结直肠癌（CRC）风险增加之间的关系。人IGF-1基因由6个外显子组成，并表现出mRNA的6种不同剪接变体（同种型）（IA、IB、IC、IIA、IIB和IIC）的表达。本研究的目的是评估IGF-1 mRNA的不同亚型在结直肠癌和正常结肠组织中的表达。采用定量聚合酶链反应（PCR）和免疫细胞化学（IHC）方法对13例配对组织标本（结直肠肿瘤和非肿瘤组织）进行分析。检测IGF-1 mRNA的I类、II类及A、B、C变体的表达。在CRC中，检测到IGF-1 II类mRNA的量高于I类mRNA。在A、B、C亚型中，IGF-1 mRNA的A型占优势。与对照组相比，CRC中IGF-1 I类和II类mRNA以及IGF-1变体B mRNA的量降低。在大肠癌中，检测到I类和II类的相互表达之间以及I和II亚型与A、B和C之间的显著相关性。IGF-1 mRNA亚型的表达在正常组织和CRC组织之间存在差异。即使在CRC组织中IGF-1 mRNA的所有亚型表现出彼此相关，与对照相比，所有转录物（亚型A除外）的表达均显著降低。
Several epidemiological studies have attempted to demonstrate a relationship between increased serum level of insulin-like growth factor 1 (IGF-1) and an augmented risk of developing colorectal cancers (CRC). The human IGF-1 gene is composed of 6 exons and demonstrated expression of 6 different splice variants (isoforms) of mRNA (IA, IB, IC, IIA, IIB and IIC). The aim of the study was to evaluate the expression of different isoforms of IGF-1 mRNA in CRC and normal colon tissue. 13 paired tissue specimens (colorectal tumor and non-tumor tissues) were analyzed using both quantitative polymerase chain reaction (PCR) and immunocytochemistry methods (IHC). The expression of classes I and II and variants A, B, C of IGF-1 mRNA were measured. In CRC higher amounts of IGF-1 class II mRNA than class I mRNA were detected. Among A, B, C isoforms, A variant of IGF-1 mRNA prevailed. The amounts of IGF-1 class I and class II mRNAs and of IGF-1 variant B mRNA were lowered in CRC as compared to the control. In CRC significant correlations were detected between reciprocal expression of class I and class II as well as between I and II isoforms and A, B and C. Expression of IGF-1 mRNA isoforms differs between normal and CRC tissues. Even if all isoforms of IGF-1 mRNA manifested correlations with each other in tissues of CRC, expression of all transcripts (except that of isoform A) was significantly decreased as compared to the control.
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影响因子：
--
作者：
Tan DS;Cook A;Chew SL
通讯作者：
Chew SL
影响因子：
50.5
作者：
Boyle, P;Ferlay, J
通讯作者：
Ferlay, J
影响因子：
2.2
作者：
Jehle, PM;Fussgaenger, RD;Jungwirth, RJ
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影响因子：
2.7
作者：
Koczorowska, Maria Magdalena;Kwasniewska, Anna;Gozdzicka-Jozefiak, Anna
通讯作者：
Gozdzicka-Jozefiak, Anna
影响因子：
1.4
作者：
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通讯作者：
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