Nucleolar localization of an isoform of the IGF-I precursor.
Nucleolar localization of an isoform of the IGF-I precursor.
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DOI：
10.1186/1471-2121-3-17
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发表时间：
2002-07-02
期刊：
影响因子：
--
通讯作者：
Chew SL
中科院分区：
文献类型：
--
作者：
Tan DS;Cook A;Chew SL
替代外显子编码人胰岛素样生长因子-I（IGF-I）前体的不同亚型，而不改变成熟IGF-I。我们假设不同的IGF-I前体可能以不同的方式运输IGF-I。嵌合IGF-I前体用克隆在信号和成熟IGF-I结构域之间的绿色荧光蛋白（GFP）制备。含有外显子1或2的嵌合体位于细胞质中，与分泌途径一致，并表明这两个外显子编码功能信号肽。含有外显子5的嵌合体定位于细胞核并强烈定位于核仁，而含有外显子6或外显子5上游部分的嵌合体则不然。当成熟的IGF-I结构域从嵌合体中缺失或信号肽缺失时，也发生核和核仁定位。我们已经确定了人IGF-I前体亚型的核仁定位。这些发现与位于外显子5编码结构域的C-末端部分的核和核仁定位信号的存在是一致的，与其他几种生长因子中的信号相似。
Alternative exons encode different isoforms of the human insulin-like growth factor-I (IGF-I) precursor without altering mature IGF-I. We hypothesized that the various IGF-I precursors may traffic IGF-I differently. Chimeric IGF-I precursors were made with green fluorescent protein (GFP) cloned between the signal and mature IGF-I domains. Chimeras containing exons 1 or 2 were located in the cytoplasm, consistent with a secretory pathway, and suggesting that both exons encoded functional signal peptides. Exon 5-containing chimeras localized to the nucleus and strongly to the nucleolus, while chimeras containing exon 6 or the upstream portion of exon 5 did not. Nuclear and nucleolar localization also occurred when the mature IGF-I domain was deleted from the chimeras, or when signal peptides were deleted. We have identified a nucleolar localization for an isoform of the human IGF-I precursor. The findings are consistent with the presence of a nuclear and nucleolar localization signal situated in the C-terminal part of the exon 5-encoded domain with similarities to signals in several other growth factors.
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影响因子：
5.3
作者：
Reimers, K;Antoine, M;Kiefer, P
通讯作者：
Kiefer, P
影响因子：
5.3
作者：
Weber, JD;Kuo, ML;Sherr, CJ
通讯作者：
Sherr, CJ
DOI：
10.1083/jcb.150.3.433
发表时间：
2000-08-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dundr M;Misteli T;Olson MO
通讯作者：
Olson MO
影响因子：
11.4
作者：
KIEFER, P;ACLAND, P;DICKSON, C
通讯作者：
DICKSON, C
影响因子：
14.9
作者：
Pederson, T
通讯作者：
Pederson, T