Revealing the characteristics of ZIKV infection through tissue-specific transcriptome sequencing analysis.
Revealing the characteristics of ZIKV infection through tissue-specific transcriptome sequencing analysis.
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通过组织特异性转录组测序分析揭示ZIKV感染特征
DOI：
10.1186/s12864-022-08919-5
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发表时间：
2022-10-08
期刊：
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
最近，寨卡病毒（ZIKV）在印度重新出现，并可能与小头畸形症有关。然而，ZIKV发病机制的分子机制仍有待探索。在本文中，我们对ZIKV感染的JEG-3、U-251 MG和HK-2细胞相对于相应的未感染对照进行了全面的RNA测序分析。结合一系列功能分析，包括基因注释、途径富集和蛋白质-蛋白质相互作用（PPI）网络分析，我们确定了ZIKV感染在不同组织和侵袭时间点诱导的分子特征。数据显示，ZIKV在每个易感器官中的感染和复制通常刺激干扰素产生并下调代谢相关过程。此外，ZIKV感染后诱导组织特异性免疫应答或生物过程（BP），包括JEG-3细胞中的GnRH信号通路、U-251 MG细胞中的MAPK信号通路和HK-2细胞中的PPAR信号通路。值得注意的是，ZIKV感染在胎盘来源的细胞系中诱导了延迟的抗病毒干扰素应答，这可能解释了ZIKV在胎盘中快速复制并发生间接垂直传播的分子机制。总之，这些数据可以提供对ZIKV感染在不同的人组织来源的细胞系中的发病机制的系统性洞察，这可能有助于开发针对ZIKV感染的预防和治疗策略。在线版本包含补充材料，可通过10.1186/s12864-022-08919-5获得。
Recently, Zika virus (ZIKV) re-emerged in India and was potentially associated with microcephaly. However, the molecular mechanisms underlying ZIKV pathogenesis remain to be explored. Herein, we performed a comprehensive RNA-sequencing analysis on ZIKV-infected JEG-3, U-251 MG, and HK-2 cells versus corresponding uninfected controls. Combined with a series of functional analyses, including gene annotation, pathway enrichment, and protein–protein interaction (PPI) network analysis, we defined the molecular characteristics induced by ZIKV infection in different tissues and invasion time points. Data showed that ZIKV infection and replication in each susceptible organ commonly stimulated interferon production and down-regulated metabolic-related processes. Also, tissue-specific immune responses or biological processes (BPs) were induced after ZIKV infection, including GnRH signaling pathway in JEG-3 cells, MAPK signaling pathway in U-251 MG cells, and PPAR signaling pathway in HK-2 cells. Of note, ZIKV infection induced delayed antiviral interferon responses in the placenta-derived cell lines, which potentially explains the molecular mechanism by which ZIKV replicates rapidly in the placenta and subsequential vertical transmission occurs. Together, these data may provide a systemic insight into the pathogenesis of ZIKV infection in distinct human tissue-derived cell lines, which is likely to help develop prophylactic and therapeutic strategies against ZIKV infection. The online version contains supplementary material available at 10.1186/s12864-022-08919-5.
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影响因子：
5.7
作者：
Baloch Z;Shen Z;Zhang L;Feng Y;Li D;Zhang NN;Deng YQ;Yang C;Sun X;Dai J;Yang Z;Qin CF;Xia X
通讯作者：
Xia X
影响因子：
3.8
作者：
Faye O;Freire CC;Iamarino A;Faye O;de Oliveira JV;Diallo M;Zanotto PM;Sall AA
通讯作者：
Sall AA
DOI：
10.1056/nejmoa1602412
发表时间：
2016-12-15
期刊：
The New England journal of medicine
影响因子：
--
作者：
Brasil P;Pereira JP Jr;Moreira ME;Ribeiro Nogueira RM;Damasceno L;Wakimoto M;Rabello RS;Valderramos SG;Halai UA;Salles TS;Zin AA;Horovitz D;Daltro P;Boechat M;Raja Gabaglia C;Carvalho de Sequeira P;Pilotto JH;Medialdea-Carrera R;Cotrim da Cunha D;Abreu de Carvalho LM;Pone M;Machado Siqueira A;Calvet GA;Rodrigues Baião AE;Neves ES;Nassar de Carvalho PR;Hasue RH;Marschik PB;Einspieler C;Janzen C;Cherry JD;Bispo de Filippis AM;Nielsen-Saines K
通讯作者：
Nielsen-Saines K
影响因子：
16.6
作者：
Chen, Qian;Gouilly, Jordi;Jabrane-Ferrat, Nabila
通讯作者：
Jabrane-Ferrat, Nabila
影响因子：
16.6
作者：
Hatton CF;Botting RA;Dueñas ME;Haq IJ;Verdon B;Thompson BJ;Spegarova JS;Gothe F;Stephenson E;Gardner AI;Murphy S;Scott J;Garnett JP;Carrie S;Powell J;Khan CMA;Huang L;Hussain R;Coxhead J;Davey T;Simpson AJ;Haniffa M;Hambleton S;Brodlie M;Ward C;Trost M;Reynolds G;Duncan CJA
通讯作者：
Duncan CJA