Molecular evolution of Zika virus during its emergence in the 20(th) century.
Molecular evolution of Zika virus during its emergence in the 20(th) century.
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DOI：
10.1371/journal.pntd.0002636
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发表时间：
2014
影响因子：
3.8
通讯作者：
Sall AA
中科院分区：
文献类型：
--
作者：
Faye O;Freire CC;Iamarino A;Faye O;de Oliveira JV;Diallo M;Zanotto PM;Sall AA
寨卡病毒（ZIKV）是一种蚊媒黄病毒，于1947年首次在乌干达分离出来。尽管昆虫学和病毒学监测报告了非洲和亚洲不同国家的ZIKV地方病活动，但直到2007年密克罗尼西亚发生寨卡热流行之前，很少报告人类病例。在西非的背景下，达喀尔巴斯德研究所的世卫组织虫媒病毒和出血热合作中心（http：//www.pasteur.fr/recherche/banques/CRORA/）报告了自1968年以来ZIKV的周期性传播。尽管有几份关于ZIKV的报告，但西非病毒株之间的遗传关系仍然知之甚少。为了评估病毒传播及其分子流行病学，我们调查了1968年至2002年在塞内加尔和科特迪瓦的六个地方收集的37株ZIKV分离株。此外，我们还包括来自其他六个国家的菌株。我们的结果表明，西非的这两个国家在世纪经历了至少两次ZIKV的独立引入，并且显然这些病毒谱系不受蚊子媒介物种的限制。此外，我们提出的证据表明，ZIKV在自然界中可能经历了重组，并且包膜蛋白中N154糖基化位点的丢失是对达氏伊蚊载体的可能适应性反应。寨卡热是一种由黄病毒引起的蚊媒疾病。人类感染寨卡病毒（ZIKV）可导致发热、不适和皮疹。尽管自1947年首次在乌干达的寨卡森林中分离出ZIKV以来有过几次报道，但ZIKV作为一种新兴病原体的分子进化仍然知之甚少。此外，尽管有来自非洲和亚洲的几起ZIKV报告，但直到2007年密克罗尼西亚发生流行病时，才报告了很少的人间病例。自1968年以来，西非的监测项目报告了该病毒的周期性传播。为了帮助填补理解ZIKV进化的差距，分析了43个ZIKV样品。我们的重点是：（i）适应性遗传变化，包括蛋白质糖基化模式，（ii）分离株之间的系统发育关系及其在非洲和亚洲传播的时空模式，（iii）脊椎动物宿主和无脊椎动物媒介物种之间的分散。我们的研究结果表明，ZIKV可能在自然界中经历了重组，并且在世纪初从乌干达出现后，它在世纪上半叶转移到西非和亚洲，对宿主和媒介物种没有任何明显的偏好。
Zika virus (ZIKV) is a mosquito-borne flavivirus first isolated in Uganda in 1947. Although entomological and virologic surveillance have reported ZIKV enzootic activity in diverse countries of Africa and Asia, few human cases were reported until 2007, when a Zika fever epidemic took place in Micronesia. In the context of West Africa, the WHO Collaborating Centre for Arboviruses and Hemorrhagic Fever at Institut Pasteur of Dakar (http://www.pasteur.fr/recherche/banques/CRORA/) reports the periodic circulation of ZIKV since 1968. Despite several reports on ZIKV, the genetic relationships among viral strains from West Africa remain poorly understood. To evaluate the viral spread and its molecular epidemiology, we investigated 37 ZIKV isolates collected from 1968 to 2002 in six localities in Senegal and Côte d'Ivoire. In addition, we included strains from six other countries. Our results suggested that these two countries in West Africa experienced at least two independent introductions of ZIKV during the 20th century, and that apparently these viral lineages were not restricted by mosquito vector species. Moreover, we present evidence that ZIKV has possibly undergone recombination in nature and that a loss of the N154 glycosylation site in the envelope protein was a possible adaptive response to the Aedes dalzieli vector. Zika fever is a mosquito-borne illness caused by a flavivirus. Human infections with Zika virus (ZIKV) could cause fever, malaise and cutaneous rash. Despite several ZIKV reports since 1947 when it was first isolated at Zika forest in Uganda, molecular evolution of ZIKV as an emerging agent remains poorly understood. Moreover, despite several ZIKV reports from Africa and Asia, few human cases were notified until 2007 when an epidemic took place in Micronesia. In West Africa, surveillance programs have reported periodic circulation of the virus since 1968. To help fill the gap in understanding ZIKV evolution, 43 ZIKV samples were analyzed. We focused on: (i) adaptive genetic changes including protein glycosylation patterns, (ii) phylogenetic relationship among isolates and their spatiotemporal patterns of spread across Africa and Asia and, (iii) dispersion among vertebrate reservoirs and invertebrate vector species. Our results indicated that ZIKV may have experienced recombination in nature and that, after it emerged from Uganda in the early of the 20th century, it moved to West Africa and Asia in the first half of the century, without any clear preference for host and vector species.
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影响因子：
11.8
作者：
Foy BD;Kobylinski KC;Chilson Foy JL;Blitvich BJ;Travassos da Rosa A;Haddow AD;Lanciotti RS;Tesh RB
通讯作者：
Tesh RB
影响因子：
8.8
作者：
Faye, Oumar;Faye, Ousmane;Sall, Amadou Alpha
通讯作者：
Sall, Amadou Alpha
DOI：
10.1016/s0923-2516(06)80135-4
发表时间：
1992-11-01
期刊：
RESEARCH IN VIROLOGY
影响因子：
--
作者：
DIGOUTTE, JP;CALVOWILSON, MA;ADAM, F
通讯作者：
ADAM, F
影响因子：
--
作者：
FAGBAMI, AH
通讯作者：
FAGBAMI, AH
影响因子：
9.8
作者：
Drummond AJ;Ho SY;Phillips MJ;Rambaut A
通讯作者：
Rambaut A