Intraspinal microstimulation produces over-ground walking in anesthetized cats.
Intraspinal microstimulation produces over-ground walking in anesthetized cats.
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DOI：
10.1088/1741-2560/13/5/056016
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发表时间：
2016-10
影响因子：
4
通讯作者：
Mushahwar VK
中科院分区：
文献类型：
--
作者：
Holinski BJ;Mazurek KA;Everaert DG;Toossi A;Lucas-Osma AM;Troyk P;Etienne-Cummings R;Stein RB;Mushahwar VK
脊髓损伤导致运动、感觉和自主神经功能的急剧丧失。本项目的目的是研究使用椎管内微刺激（ISMS）产生长距离的地面行走。ISMS是一种电刺激方法，通过激活损伤水平以下的脊髓网络来恢复运动功能。它通过使用细穿透电极（直径≤ 50µm）刺激腰膨大的腹角来产生腿部运动。在5只成年猫（4.2-5.5kg）中，通过植入16个电极的ISMS施加，电极尖端靶向双侧腹角中的第IX层。桌面系统实现了基于生理学的控制策略，该控制策略通过电极组递送不同的刺激模式，以利用对应于摆动和站立的适当的肢体运动学和力来引起行走运动。每只猫都走过一条装有仪器的2.9 m走道，并记录肢体运动学和力。地面行走需要推进力和支撑力。三只动物的累积行走距离范围为609 m至835 m（最长试验距离）。在这三只猫中，平均峰值支撑力为3.5±0.6N，对应于后腿的全重量支撑，而髋关节、膝关节和踝关节的角度范围分别为23.1±2.0°、29.1±0.2°和60.3±5.2°。为了进一步证明ISMS在未来临床应用中的可行性，在试验子集中成功实施了原型植入式模块，并产生了相当的行走性能。通过激活腰骶脊髓内的固有运动网络，ISMS能够产生电流幅度<100 µA的双侧协调和功能性地面行走。这些令人兴奋的结果表明，ISMS可能是恢复脊髓损伤后功能性行走的有效干预措施。
Spinal cord injury causes a drastic loss of motor, sensory and autonomic function. The goal of this project was to investigate the use of intraspinal microstimulation (ISMS) for producing long distances of walking over ground. ISMS is an electrical stimulation method developed for restoring motor function by activating spinal networks below the level of an injury. It produces movements of the legs by stimulating the ventral horn of the lumbar enlargement using fine penetrating electrodes (≤ 50µm diameter). In each of five adult cats (4.2–5.5kg), ISMS was applied through 16 electrodes implanted with tips targeting lamina IX in the ventral horn bilaterally. A desktop system implemented a physiologically-based control strategy that delivered different stimulation patterns through groups of electrodes to evoke walking movements with appropriate limb kinematics and forces corresponding to swing and stance. Each cat walked over an instrumented 2.9m walkway and limb kinematics and forces were recorded. Both propulsive and supportive forces were required for over-ground walking. Cumulative walking distances ranging from 609m to 835m (longest tested) were achieved in three animals. In these three cats, the mean peak supportive force was 3.5±0.6N corresponding to full-weight-support of the hind legs, while the angular range of the hip, knee, and ankle joints were 23.1±2.0°, 29.1±0.2°, and 60.3±5.2°, respectively. To further demonstrate the viability of ISMS for future clinical use, a prototype implantable module was successfully implemented in a subset of trials and produced comparable walking performance. By activating inherent locomotor networks within the lumbosacral spinal cord, ISMS was capable of producing bilaterally coordinated and functional over-ground walking with current amplitudes <100 µA. These exciting results suggest that ISMS may be an effective intervention for restoring functional walking after spinal cord injury.
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影响因子：
64.8
作者：
Ethier, C.;Oby, E. R.;Bauman, M. J.;Miller, L. E.
通讯作者：
Miller, L. E.
影响因子：
3
作者：
Brissot, R;Gallien, P;Dassonville, J
通讯作者：
Dassonville, J
影响因子：
14
作者：
Bamford, Jeremy A.;Todd, Kathryn G.;Mushahwar, Vivian K.
通讯作者：
Mushahwar, Vivian K.
影响因子：
5.1
作者：
Bulea, Thomas C.;Kobetic, Rudi;Triolo, Ronald J.
通讯作者：
Triolo, Ronald J.
影响因子：
--
作者：
Bamford, Jeremy A.;Mushahwar, Vivian K.
通讯作者：
Mushahwar, Vivian K.