Vancomycin forms ligand-mediated supramolecular complexes.
Vancomycin forms ligand-mediated supramolecular complexes.
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DOI：
10.1016/j.jmb.2008.10.049
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发表时间：
2009-01-09
影响因子：
5.6
通讯作者：
Axelsen, Paul H.
中科院分区：
文献类型：
--
作者：
Loll, Patrick J.;Derhovanessian, Ariss;Shapovalov, Maxim V.;Kaplan, Jeffrey;Yang, Lin;Axelsen, Paul H.
关键词：
The emergence of resistance to vancomycin and related glycopeptide antibiotics is spurring efforts to develop new antimicrobial therapeutics. High resolution structural information about antibiotic-ligand recognition should prove valuable in the rational design of improved drugs. We have determined the X-ray crystal structure of the complex of vancomycin with N-acetyl-D-Ala-D-Ala, a mimic of the natural muramyl peptide target, and refined this structure at a resolution of 1.3 Å to R and Rfree values of 0.172 and 0.195, respectively. The crystal asymmetric unit contains three back-back vancomycin dimers; two of these dimers participate in ligand-mediated face-face interactions that produce an infinite chain of molecules running throughout the crystal. The third dimer packs against the side of a face-face interface in a tight “side-side” interaction that involves both polar contacts and burial of hydrophobic surface. The trimer of dimers found in the asymmetric unit is essentially identical to complexes seen in three other crystal structures of glycopeptide antibiotics complexed with peptide ligands. These four structures are derived from crystals belonging to different space groups, suggesting that the trimer of dimers may not be simply a crystal packing artifact, and prompting us to ask if ligand-mediated oligomerization could be observed in solution. Using size exclusion chromatography, dynamic light scattering, and small-angle X-ray scattering, we demonstrate that vancomycin forms discrete supramolecular complexes in the presence of tripeptide ligands. Size estimates for these complexes are consistent with assemblies containing 4-6 vancomycin monomers.
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