Two chronic myelogenous leultaemia cell lines which represent different stages of erythroid differentiation
Two chronic myelogenous leultaemia cell lines which represent different stages of erythroid differentiation
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代表红系分化不同阶段的两种慢性粒细胞白血病细胞系
DOI：
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发表时间：
1993
影响因子：
6.5
通讯作者：
K. Abe
中科院分区：
文献类型：
--
作者：
K. Endo;H. Harigae;T. Nagai;H. Fujie;K. Meguro;N. Watanabe;K. Furuyama;J. Kameoka;M. Okuda;N. Hayashi;Masayuki Yamamoto;K. Abe
我们建立了两种细胞系，YN-1和Y-1 K。从两名慢性粒细胞白血病急变期患者的外周血中提取。YN-1和Y-1 K细胞的表征揭示了这些细胞表达红系谱系标志物。然而，YN-1和Y-1 K细胞中γ-珠蛋白mRNA和血红蛋白的水平存在显著差异。YN-1含有约1-5%联苯胺阳性染色细胞，而在Y-1 K细胞中未观察到联苯胺阳性细胞。各种红系分化的化学诱导剂显著增加了YN-1细胞中的血红蛋白产量，但Y-1 K细胞中没有。相反，Y-1 K细胞表达CD 34干细胞抗原和CD 41巨核细胞特异性抗原。这些观察结果表明，尽管两种细胞系都定型为红系谱系。每种细胞系代表红细胞分化程序中的不同分化阶段。Y-1 K似乎对应于红系细胞的早期阶段，而YN-1代表人类红细胞生成的更晚期阶段。
Summary We established two cell lines, YN‐1 and Y‐1K. from the peripheral blood of two chronic myelogenous leukaemia patients in blastic crisis. Characterization of the YN‐1 and Y‐1K cells revealed that these cells expressed erythroid lineage markers. However, there was a marked difference in the level of γ‐globin mRNA and haenioglobin in YN‐1 and Y‐1K cells. YN‐1 contained approximately 1–5% benzidine‐positive staining cells, whereas no benzidine‐positive cells were observed in Y‐1K cells. Haemoglobin production in YN‐1 cells was markedly increased with various chemical inducers of erythroid differentiation, but was not in Y‐1K cells. In contrast, Y‐1K cells expressed CD34 stem cell antigen and CD41 megakaryocyte‐specific antigen. These observations suggested that, although both cell lines were committed to the erythroid lineage. each cell line represented a distinct differentiation stage in the erythroid differentiation programme. Y‐1K seemed to correspond to an early stage of cells in erythroid lineage, whereas YN‐1 represented a more advanced stage in human erythropoiesis.
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影响因子：
56.9
作者：
MARTIN, P;PAPAYANNOPOULOU, T
通讯作者：
PAPAYANNOPOULOU, T
两种新的人类红白血病系的表面抗原谱和球蛋白表型：特征和解释。
影响因子：
20.3
作者：
Papayannopoulou,T;Nakamoto,B;Kurachi,S;Tweeddale,M;Messner,H
通讯作者：
Messner,H
影响因子：
20.3
作者：
Richard E. Champlin;David W. Golde
通讯作者：
Richard E. Champlin;David W. Golde
嵌合 BCR-abl 信使 RNA 作为费城染色体阳性急性淋巴细胞白血病移植患者微小残留病的标志物。
影响因子：
20.3
作者：
Gehly,GB;Bryant,EM;Lee,AM;Kidd,PG;Thomas,ED
通讯作者：
Thomas,ED
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fujita,H;Yamamoto,M;Yamagami,T;Hayashi,N;Sassa,S
通讯作者：
Sassa,S