Rate and Extent of Growth of a Model Extremophile, Archaeoglobus fulgidus, Under High Hydrostatic Pressures
Rate and Extent of Growth of a Model Extremophile, Archaeoglobus fulgidus, Under High Hydrostatic Pressures
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高静水压下嗜极微生物模型的生长速率和程度，古生球菌 fulgidus
DOI：
10.3389/fmicb.2020.01023
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发表时间：
2020
影响因子：
5.2
通讯作者：
K. Rogers
中科院分区：
文献类型：
--
作者：
G. Oliver;A. Cario;K. Rogers
对一株模式极端微生物--闪烁古生球菌VC-16进行了高静水压分批培养，以探讨高压对深海生物圈微生物生长和生理的影响。虽然通常在高温高压海洋环境（海平面以下2-5 km，20-50 MPa压力）中识别，但A。以前没有表征过在高压下的闪烁线生长。这里，A. fulgidus被观察到高达60 MPa时，由乳酸氧化耦合硫酸盐还原的异养代谢的支持，和高达40 MPa的自养CO2固定耦合硫代硫酸盐还原通过H2。在20 MPa下，异养代谢的最大生长速率被观察到，这表明A。在这些条件下，闪电鱼是中等的嗜压生物。然而，只有piezotolerance观察到的自养，生长速率几乎保持不变，从0.3至40 MPa。下面描述的实验表明，A. fulgidus继续异养硫酸盐还原和自养硫代硫酸盐还原几乎不受增加的压力分别高达30 MPa和40 MPa。由于这些压力包括各种地下海洋环境，A。闪电菌是探索高压对深层地下微生物代谢影响的嗜极微生物模型。此外，这些结果表明需要高压培养深海和地下微生物，以更好地反映原位生理条件。
High hydrostatic pressure (HHP) batch cultivation of a model extremophile, Archaeoglobus fulgidus type strain VC-16, was performed to explore how elevated pressures might affect microbial growth and physiology in the deep marine biosphere. Though commonly identified in high-temperature and high-pressure marine environments (up to 2–5 km below sea level, 20–50 MPa pressures), A. fulgidus growth at elevated pressure has not been characterized previously. Here, exponential growth of A. fulgidus was observed up to 60 MPa when supported by the heterotrophic metabolism of lactate oxidation coupled to sulfate reduction, and up to 40 MPa for autotrophic CO2 fixation coupled to thiosulfate reduction via H2. Maximum growth rates for this heterotrophic metabolism were observed at 20 MPa, suggesting that A. fulgidus is a moderate piezophile under these conditions. However, only piezotolerance was observed for autotrophy, as growth rates remained nearly constant from 0.3 to 40 MPa. Experiments described below show that A. fulgidus continues both heterotrophic sulfate reduction and autotrophic thiosulfate reduction nearly unaffected by increasing pressure up to 30 MPa and 40 MPa, respectively. As these pressures encompass a variety of subsurface marine environments, A. fulgidus serves as a model extremophile for exploring the effects of elevated pressure on microbial metabolisms in the deep subsurface. Further, these results exemplify the need for high-pressure cultivation of deep-sea and subsurface microorganisms to better reflect in situ physiological conditions.
影响因子：
64.8
作者：
Santelli, Cara M.;Orcutt, Beth N.;Edwards, Katrina J.
通讯作者：
Edwards, Katrina J.
Archaeoglobus sulfaticallidus sp nov，一种嗜热且兼性岩石自养硫酸盐还原剂，从暴露于热脊侧地壳流体的黑锈中分离出来
DOI：
10.1099/ijs.0.016105-0
发表时间：
2010
影响因子：
2.8
作者：
Steinsbu BO;Thorseth IH;Nakagawa S;Inagaki F;Lever MA;Engelen B;Øvreås L;Pedersen RB
通讯作者：
Pedersen RB