Archaeoglobus sulfaticallidus sp. nov., a thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids.

Archaeoglobus sulfaticallidus sp. nov., a thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids.
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Archaeoglobus sulfaticallidus sp nov,一种嗜热且兼性岩石自养硫酸盐还原剂,从暴露于热脊侧地壳流体的黑锈中分离出来

DOI:
10.1099/ijs.0.016105-0
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发表时间:
2010
影响因子:
2.8
通讯作者:
Pedersen RB
Pedersen RB
中科院分区:
生物学3区
文献类型:
--
作者:
Steinsbu BO;Thorseth IH;Nakagawa S;Inagaki F;Lever MA;Engelen B;Øvreås L;Pedersen RB

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从东太平洋Juan de Fuca Ridge东侧IODP探险期间发现的一个钻孔(Cork 1026b)的钢质表面形成的黑色锈菌中分离到一种新的嗜热自养硫酸盐还原古生物。该菌株的细胞呈瓣状或三角形。最适生长温度为75 °C,最适pH为7,最适氯化钠浓度为2 %(w/v)。该菌株严格厌氧,以硫酸盐、亚硫酸盐或硫代硫酸盐为电子受体,在H_2和CO_2上自养生长。乳酸和丙酮酸可以作为替代能源和碳源。基因组G+C含量为42 摩尔%。16S rRNA基因的系统发育分析表明,该分离物与古球菌科成员亲缘关系较近,序列相似性为90.3-94.4 %。生理和分子生物学特性表明,该分离物为古球藻属的一个新种。名称:硫化古陆藻属(Archaeoglobus Sulami Callidus)11月模式菌株为PM70-1T(=DSM 19444T=JCM 14716T)。
A novel thermophilic and lithoautotrophic sulfate-reducing archaeon was isolated from black rust formed on the steel surface of a borehole observatory (CORK 1026B) retrieved during IODP Expedition 301 on the eastern flank of Juan de Fuca Ridge, eastern Pacific Ocean. Cells of the strain were lobe-shaped or triangular. The optimum temperature, pH and NaCl concentration for growth were 75 °C, pH 7 and 2 % (w/v), respectively. The isolate was strictly anaerobic, growing lithoautotrophically on H2and CO2using sulfate, sulfite or thiosulfate as electron acceptors. Lactate and pyruvate could serve as alternative energy and carbon sources. The G+C content of the genomic DNA was 42 mol%. Phylogenetic analyses of the 16S rRNA gene indicated that the isolate was closely related to members of the familyArchaeoglobaceae, with sequence similarities of 90.3–94.4 %. Physiological and molecular properties showed that the isolate represents a novel species of the genusArchaeoglobus. The nameArchaeoglobus sulfaticallidussp. nov. is proposed; the type strain is PM70-1T(=DSM 19444T=JCM 14716T).
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