Decoding Huge Phage Diversity: A Taxonomic Classification of Lak Megaphages
Decoding Huge Phage Diversity: A Taxonomic Classification of Lak Megaphages
复制标题
解码巨大的噬菌体多样性：Lak 巨噬细胞的分类学
DOI：
10.1101/2024.02.01.578382
复制
发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Cook R
中科院分区：
文献类型：
--
作者：
Cook R
对未培养病毒的高通量测序加速了对全球病毒多样性的理解，并发现了比迄今培养的任何病毒都大得多的病毒基因组。值得注意的是，LAK噬菌体是一组神秘的病毒，呈现了在人类和动物微生物群中已知的一些最大的噬菌体基因组，与任何培养的病毒不同。尽管测序数据集中存在着丰富的病毒多样性，但未培养的病毒很少被用于分类。我们研究了23个LAK噬菌体的进化关系，并提出了分类方法。预测的蛋白质分析显示，LAK噬菌体在尾状病毒类中形成了一个深分枝的单系分支，不包含其他噬菌体基因组。这个分支的一个有趣的特征是，所有当前的成员都具有另一种遗传密码。我们认为LAK噬菌体属于一个新目，即‘Grandevirales’。基于蛋白质和核苷酸的分析支持在Grandevirales目中创建两个家族、三个亚家族和四个属。我们预计，拟议的LAK巨噬细胞分类法将简化未来发现的相关病毒基因组的分类。继续努力对不同的病毒进行分类，对于帮助对病毒基因组和后基因组进行共同分析至关重要。
High-throughput sequencing for uncultivated viruses has accelerated the understanding of global viral diversity and uncovered viral genomes substantially larger than any that have so far been cultured. Notably, the Lak phages are an enigmatic group of viruses that present some of the largest known phage genomes identified in human and animal microbiomes, and are dissimilar to any cultivated viruses. Despite the wealth of viral diversity that exists within sequencing datasets, uncultivated viruses have rarely been used for taxonomic classification. We investigated the evolutionary relationships of 23 Lak phages and propose a taxonomy for their classification. Predicted protein analysis revealed the Lak phages formed a deeply branching monophyletic clade within the classCaudoviriceteswhich contained no other phage genomes. One of the interesting features of this clade is that all current members are characterised by an alternative genetic code. We propose the Lak phages belong to a new order, the ‘Grandevirales’. Protein and nucleotide-based analyses support the creation of two families, three sub-families, and four genera within the order ‘Grandevirales’. We anticipate that the proposed taxonomy of Lak megaphages will simplify the future classification of related viral genomes as they are uncovered. Continued efforts to classify divergent viruses are crucial to aid common analyses of viral genomes and metagenomes.
登录
查看更多内容
影响因子：
9.2
作者：
Prasoodanan P K V;Sharma AK;Mahajan S;Dhakan DB;Maji A;Scaria J;Sharma VK
通讯作者：
Sharma VK
影响因子：
3.8
作者：
Postler, Thomas S.;Rubino, Luisa;Adriaenssens, Evelien M.;Dutilh, Bas E.;Harrach, Balazs;Junglen, Sandra;Kropinski, Andrew M.;Krupovic, Mart;Wada, Jiro;Crane, Anya;Kuhn, Jens H.;Mushegian, Arcady;Rumnieks, Janis;Sabanadzovic, Sead;Simmonds, Peter;Varsani, Arvind;Zerbini, F. Murilo;Callanan, Julie;Draper, Lorraine A.;Hill, Colin;Stockdale, Stephen R.
通讯作者：
Stockdale, Stephen R.
影响因子：
4.6
作者：
Terzian P;Olo Ndela E;Galiez C;Lossouarn J;Pérez Bucio RE;Mom R;Toussaint A;Petit MA;Enault F
通讯作者：
Enault F
影响因子：
3.4
作者：
Hitch, Thomas C. A.;Bisdorf, Kevin;Clavel, Thomas
通讯作者：
Clavel, Thomas
DOI：
10.1089/phage.2021.0007
发表时间：
2021-12-01
期刊：
PHAGE-THERAPY APPLICATIONS AND RESEARCH
影响因子：
--
作者：
Cook, Ryan;Brown, Nathan;Millard, Andrew
通讯作者：
Millard, Andrew