Fibulin-3 knockdown inhibits cervical cancer cell growth and metastasis in vitro and in vivo.
Fibulin-3 knockdown inhibits cervical cancer cell growth and metastasis in vitro and in vivo.
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Fibulin-3 敲低可抑制宫颈癌细胞的体外和体内生长和转移
DOI：
10.1038/s41598-018-28906-9
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发表时间：
2018-07-13
影响因子：
4.6
通讯作者：
Shi M
中科院分区：
文献类型：
--
作者：
Li J;Qi C;Liu X;Li C;Chen J;Shi M
为探讨fibulin-3在宫颈癌恶性细胞生长和转移中的作用，采用免疫组化方法检测fibulin-3在正常宫颈组织、宫颈上皮内瘤样病变（CIN）和宫颈癌中的表达。定量实时聚合酶链反应，蛋白质印迹，免疫细胞化学进行评估fibulin-3的表达在mRNA和蛋白质水平在不同的侵袭性克隆亚系。用fibulin-3shRNA和fibulin-3cDNA检测强、弱侵袭性克隆亚系。采用体外和体内功能测定，我们研究了下调和上调fibulin-3表达对不同克隆亚系增殖和侵袭的影响。在慢病毒转染系统中，对上皮间质转化（EMT）及其信号通路PI3K/AKT和ERK进行了研究。Fibulin-3在宫颈癌组织中表达上调，其过表达与宫颈癌的恶性表型及不良预后密切相关。Fibulin-3通过诱导EMT和激活PI3 K-Akt-mTOR信号转导通路促进宫颈癌细胞的侵袭能力。Fibulin-3可能参与宫颈癌的发生发展过程。本文的结果将有助于为宫颈癌患者开发新的预后因素和可能的治疗选择。
To explore the function of fibulin-3 in cervical carcinoma malignant cell growth and metastasis, fibulin-3 expression in normal cervical tissue, cervical intraepithelial neoplasia (CIN), and cervical carcinoma were evaluated by immunohistochemistry. Quantitative real-time-polymerase chain reaction, western blotting, and immunocytochemistry were performed to assess the expression of fibulin-3 at mRNA and protein levels in different invasive clone sublines. Fibulin-3 shRNA and fibulin-3 cDNA were used to transfect the strongly and weakly invasive clone sublines. Using in vitro and in vivo functional assays, we investigated the effects of down-regulating and up-regulating fibulin-3 expression on the proliferation and invasion of different clone sublines. Epithelial mesenchymal transition (EMT) and its signaling pathways PI3K/AKT and ERK were studied carefully in lentiviral transfection systems. Fibulin-3 was upregulated in cervical carcinoma, and its overexpression was significantly related with malignant phenotype and poor prognosis of cervical carcinoma. Fibulin-3 promoted cervical cancer cell invasive capabilities by eliciting EMT and activating the PI3K-Akt-mTOR signal transduction pathway. Fibulin-3 could facilitate the process of cervical cancer development. The results presented here will help develop novel prognostic factors and possible therapeutic options for patients with cervical cancer.
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影响因子：
82.9
作者：
通讯作者：
--
DOI：
10.4103/0971-5851.195751
发表时间：
2016-10
期刊：
Indian journal of medical and paediatric oncology : official journal of Indian Society of Medical & Paediatric Oncology
影响因子：
--
作者：
Bobdey S;Sathwara J;Jain A;Balasubramaniam G
通讯作者：
Balasubramaniam G
影响因子：
--
作者：
Yin X;Fang S;Wang M;Wang Q;Fang R;Chen J
通讯作者：
Chen J
EFEMP1通过ERK1/2活性调节MMP2和MMP9抑制肝细胞癌的迁移
影响因子：
4.2
作者：
Dou, Cheng-Yun;Cao, Chuang-Jie;Wang, Lian-Tang
通讯作者：
Wang, Lian-Tang
影响因子：
1.9
作者：
Suneja, Gita;Brown, Derek;Gaffney, David
通讯作者：
Gaffney, David