Lewy bodies are ubiquitinated
Lewy bodies are ubiquitinated
复制标题
路易体被泛素化
作者：
S. Kuzuhara;H. Mori;N. Izumiyama;M. Yoshimura;Y. Ihara
摘要使用针对细胞骨架蛋白、配对螺旋丝 (PHF) 和泛素的各种抗体，通过免疫细胞化学方法研究了 5 例弥漫性路易体病和 1 例伴有痴呆的帕金森病患者脑干和大脑皮层中路易体 (LB) 的性质。神经丝、tau 和 PHF 的 200 kDa 成分的抗体对大多数 LB 没有表现出明显的反应。高分子量微管相关蛋白 (HMWMAP) 的抗体对一些 LB 的外周进行中等程度的染色。识别泛素的 PHF 单克隆抗体 (DF2) 和泛素多克隆抗体对几乎所有典型和皮质 LB 进行了免疫染色，其强度与阿尔茨海默氏症神经原纤维缠结和老年斑神经突一样：LB 的外围被深色染色，而典型 LB 的中央核心和皮质 LB 的中心区域染色程度较低或保持未染色。带有 DF2 的 LB 的免疫电子显微镜显示，免疫反应产物仅位于 LB 外围的细丝上，而不是位于中心的细丝上。这些发现表明，尽管存在一些结构差异，两种类型的 LB 在免疫细胞化学上无法区分，并且 LB 中位于外周的细丝标记有泛素，泛素是细胞中 ATP 依赖性蛋白水解系统所需的元素。泛素抗体是已知的最有用的 LB 标记。
SummaryThe nature of Lewy bodies (LBs) in the brain stem and cerebral cortex in five cases of diffuse Lewy body disease and one case of Parkinson's disease with dementia were investigated immunocytochemically with various antibodies to cytoskeletal proteins, paired helical filaments (PHF) and ubiquitin. Antibodies to 200-kDa component of neurofilament, tau and PHF showed no significant reactions with most of LBs. Antibodies to high-molecular weight microtubule-associated proteins (HMWMAPs) moderately stained the periphery of a few of LBs. A monoclonal antibody to PHF (DF2) which recognizes ubiquitin, and polyclonal antibodies to ubiquitin immunostained virtually all of the typical and cortical LBs as intensely as Alzheimer's neurofibrillary tangles and senile plaque neurites: the periphery of LBs was darkly stained, whereas the central core of typical LBs and central zone of cortical LBs were less intensely stained or remained unstained. Immunoelectron microscopy of the LBs with DF2 revealed that immune reaction products were located on the filaments exclusively in the periphery of LBs, but not on those in the center. These findings suggest that both types of LBs are immunocytochemically indistinguishable despite some structural differences, and that peripherally located filaments in LBs are tagged with ubiquitin, an element required for the ATP-dependent proteolysis system in the cell. Antibodies to ubiquitin are the most useful marker of LBs ever known.
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影响因子：
56.9
作者：
GOLDMAN, JE;YEN, SH;PERESS, NS
通讯作者：
PERESS, NS
抗阿尔茨海默病神经原纤维缠结的单克隆抗体。
DOI：
--
发表时间：
1985
期刊：
The American journal of pathology
影响因子：
--
作者：
Dickson,DW;Kress,Y;Crowe,A;Yen,SH
通讯作者：
Yen,SH
DOI：
10.1016/s0021-9258(19)45327-1
发表时间：
1982-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Avram HershkoS;E. Eytan;A. Ciechanover;Arthur;Haass
通讯作者：
Avram HershkoS;E. Eytan;A. Ciechanover;Arthur;Haass
DOI：
10.1073/pnas.83.13.4913
发表时间：
1986-07-01
影响因子：
11.1
作者：
GRUNDKEIQBAL, I;IQBAL, K;BINDER, LI
通讯作者：
BINDER, LI