Discontinuities in the human bone-PDL-cementum complex.
Discontinuities in the human bone-PDL-cementum complex.
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DOI：
10.1016/j.biomaterials.2011.06.021
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发表时间：
2011-10
期刊：
影响因子：
14
通讯作者：
Ho SP
中科院分区：
文献类型：
--
作者：
Hurng JM;Kurylo MP;Marshall GW;Webb SM;Ryder MI;Ho SP
由于功能需求，自然分级界面可能会偏向不连续界面，最终降低动态关节的功能效率。这是一个人的纤维关节，即骨-牙齿复合体，将通过组织化学，和特定位点的高分辨率显微镜，显微断层扫描，X射线荧光成像和湿纳米压痕技术讨论的特征。结果表明，PDL间隙出现5-50 μm狭窄的两个原因：1）PDL插入部位的微观扇形区域和具有丰富嗜碱性线的宏观骨分层，以及2）宏观骨突起。狭窄的PDL-复合物说明了asporin的片状外观，并且当在潮湿条件下使用原子力显微镜（AFM）成像时，证明了PDL的结构重组、胶原蛋白周期性、PDL-牙骨质和PDL-骨附着点处以及牙骨质和骨内的有机主导区域。扫描电子显微镜（SEM）的结果证实了AFM的结果。尽管PDL变窄，但使用Micro XCT™证明了PDL与骨内膜间隙中血管系统之间的连续性。较高水平的钙和磷的X-射线荧光使用微探针与较高的弹性模量值为0.1-1.4和0.1-1.2 GPa的PDL-骨和PDL-牙骨质使用湿纳米压痕。在150-380 μm宽PDL复合体中，PDL-骨和PDL-牙骨质附着体的弹性模量值范围分别为0.1-1.0和0.1- 0.6GPa。基于这些结果，我们提出，在端点处的应变放大可以最小化，模量分布的逐渐变化，150-380 μm宽的功能PDL空间的特征。然而，模量分布的不连续性，即5-50 μm狭窄PDL空间的特征，将导致力学转导受损。骨-牙纤维关节内的狭窄或狭窄部位将成为新的“承重部位”，最终可能导致骨与牙骨质的直接局部融合。
A naturally graded interface due to functional demands can deviate toward a discontinuous interface, eventually decreasing the functional efficiency of a dynamic joint. It is this characteristic feature in a human fibrous joint i.e. bone-tooth complex that will be discussed through histochemistry, and site-specific high resolution microscopy, micro tomography, X-ray fluorescence imaging and wet nanoindentation techniques. Results demonstrated two causes for the occurrence of 5-50 μm narrowed PDL-space: 1) microscopic scalloped regions at the PDL-insertion sites and macroscale stratified layers of bone with rich basophilic lines, and 2) macroscopic bony protrusions. Narrowed PDL-complexes illustrated patchy appearance of asporin, and when imaged under wet conditions using an atomic force microscope (AFM), demonstrated structural reorganization of the PDL, collagen periodicity, organic-dominant areas at the PDL-cementum and PDL-bone entheses and within cementum and bone. Scanning electron microscopy (SEM) results confirmed AFM results. Despite the narrowed PDL, continuity between PDL and vasculature in endosteal spaces of bone was demonstrated using a Micro XCT™. The higher levels of Ca and P X-ray fluorescence using a microprobe were correlated with higher elastic modulus values of 0.1-1.4 and 0.1-1.2 GPa for PDL-bone and PDL-cementum using wet nanoindentation. The ranges in elastic modulus values for PDL-bone and PDL-cementum entheses in 150-380 μm wide PDL-complex were 0.1-1.0 and 0.1-0.6 GPa. Based on these results we propose that strain amplification at the entheses could be minimized with a gradual change in modulus profile, a characteristic of 150-380 μm wide functional PDL-space. However, a discontinuity in modulus profile, a characteristic of 5-50 μm narrowed PDL-space would cause compromised mechanotransduction. The constrictions or narrowed sites within the bone-tooth fibrous joint will become the new “load bearing sites” that eventually could cause direct local fusion of bone with cementum.
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影响因子：
4.9
作者：
Ho, SP;Balooch, M;Marshall, SJ
通讯作者：
Marshall, SJ
影响因子：
2.4
作者：
Benjamin, M;Toumi, H;Milz, S
通讯作者：
Milz, S
DOI：
10.1902/annals.2003.8.1.193
发表时间：
2003-12-01
期刊：
Annals of periodontology
影响因子：
--
作者：
Giannobile, William V;Somerman, Martha J
通讯作者：
Somerman, Martha J
影响因子：
27.4
作者：
Benjamin, M.;Toumi, H.;McGonagle, D.
通讯作者：
McGonagle, D.
影响因子：
14
作者：
Ho, Sunita P.;Kurylo, Michael P.;Fong, Tiffany K.;Lee, Stephen S. J.;Wagner, Hanoch D.;Ryder, Mark I.;Marshall, Grayson W.
通讯作者：
Marshall, Grayson W.