Virulence, Antimicrobial Susceptibility, Molecular and Epidemiological Characteristics of a New Serotype of Vibrio parahaemolyticus From Diarrhea Patients
Virulence, Antimicrobial Susceptibility, Molecular and Epidemiological Characteristics of a New Serotype of Vibrio parahaemolyticus From Diarrhea Patients
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腹泻患者副溶血性弧菌新血清型的毒力、耐药性、分子和流行病学特征
DOI：
10.3389/fmicb.2020.02025
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发表时间：
2020-08
影响因子：
5.2
通讯作者：
Yu Chen
中科院分区：
文献类型：
--
作者：
Qiaoyun Zhu;Xiao Chen;Yanchao Liu;Ruonan Wang;Jiayao Chen;Yu Chen
副溶血性弧菌是沿海地区食源性腹泻的主要病原菌。通过对1870株副溶血性弧菌的病原学特征研究，2013年以来O 4：KUT的分离率明显上升。在这项研究中，我们分析了一个新的血清型命名为O 4：KUT 2的毒力，抗菌药物敏感性，分子和流行病学特征。O 4：KUT 2菌株具有tlh+ tdh+ trh-toxRS/新特征，并在2013-2015年期间流行。91.5%的O 4：KUT 2血清型菌株对氨苄西林耐药。O 4：KUT 2菌株的生长曲线与O 4：K9、O 4：K8和O3：K6血清型菌株的生长曲线不同。O 4：KUT 2菌株属于ST 332，其中4株在recA处插入了大片段。将O 4：KUT 2株与2012年浙江省急性腹泻患者分离的同属于ST 322的O 4：K9株全基因组进行比较，在2249个位点上未发现不同的等位基因，提示O 4：KUT 2株可能来源于O 4：K9株。副溶血性弧菌O 4：KUT 2阳性患者的临床表现与O3：K6阳性患者无显著差异，尽管他们的遗传特征不同。O 4：KUT 2新血清型的出现和比例的增加提示对急性腹泻患者病原菌谱的监测不容放松。
Vibrio parahaemolyticus is the main pathogen of food-borne diarrheal in coastal areas. Through the study of pathogen characteristics of 1870 V. parahaemolyticus, the isolation rate of O4:KUT had increased significantly since 2013. In this study, we analyzed virulence, antimicrobial susceptibility, molecular, and epidemiological characteristics of a new serotype named O4:KUT2. O4:KUT2 strains had tlh+ tdh+ trh– toxRS/new– characteristics and were prevalent during 2013–2015. The 91.5% O4:KUT2 serotype strains were resistant to ampicillin. The growth curves of O4:KUT2 strains were different with O4:K9, O4:K8, and O3:K6 serotype strains. O4:KUT2 strains belonged to ST332 where four strains had a large fragment inserted at recA. Compared the whole genomes of O4:KUT2 strains with O4:K9 strain which also belonged to ST322 isolated from acute diarrhea patients in Zhejiang province in 2012, no different alleles at 2249 loci was found. This finding implied that O4:KUT2 strains might be derived from O4:K9 strains. Clinical presentation of patients positive for V. parahaemolyticus O4:KUT2 were no significant difference with patients positive for O3:K6, although their genetic characteristics were different. The appearance and the increase of proportion of the new serotype O4:KUT2 strains was aware that we should not relax the monitoring of the pathogen spectrum of acute diarrheal patients.
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影响因子：
2.8
作者：
P. Murray;E. Baron;J. Jorgensen;M. Landry;M. Pfaller
通讯作者：
P. Murray;E. Baron;J. Jorgensen;M. Landry;M. Pfaller
影响因子：
5.1
作者：
A. Klainer
通讯作者：
A. Klainer
DOI：
--
发表时间：
1953
期刊：
Medical journal of Osaka University
影响因子：
--
作者：
T. Fujino;Y. Okuno;D. Nakada;A. Aoyama;K. Fukai;T. Mukai;T. Ueho
通讯作者：
T. Fujino;Y. Okuno;D. Nakada;A. Aoyama;K. Fukai;T. Mukai;T. Ueho
影响因子：
14.2
作者：
Magiorakos, A. -P.;Srinivasan, A.;Monnet, D. L.
通讯作者：
Monnet, D. L.
影响因子：
2.6
作者：
Aroori, Sree V.;Cogan, Tristan A.;Humphrey, Tom J.
通讯作者：
Humphrey, Tom J.