A male-killing Wolbachia endosymbiont is concealed by another endosymbiont and a nuclear suppressor.
A male-killing Wolbachia endosymbiont is concealed by another endosymbiont and a nuclear suppressor.
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DOI：
10.1371/journal.pbio.3001879
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发表时间：
2023-03
期刊：
影响因子：
9.8
通讯作者：
Hoffmann, Ary
中科院分区：
文献类型：
--
作者：
Richardson, Kelly;Ross, Perran A.;Cooper, Brandon;Conner, William;Schmidt, Tom;Hoffmann, Ary
生活在昆虫宿主细胞内的细菌（内共生体）可以改变宿主的繁殖，包括杀死雄性后代（雄性杀死，MK）。MK只在少数昆虫中被描述过，但这可能反映了检测MK的挑战，而不是它的稀有性。在这里，我们确定MK沃尔巴克氏体在低频率（约4%）的自然种群的果蝇pseudotakahashii。MK Wolbachia在实验室培养过程中密度稳定，母婴传播稳定，但主要表现在幼虫期的MK表型迅速丧失。MK Wolbachia与第二种Wolbachia菌株一起发生，表达了不同的生殖操作，细胞质不相容性（CI）。基因组分析突出了Wolbachia区域在涉及17个基因的2个菌株之间的分歧，并且在Wolbachia组装中鉴定了与MK和CI有关的wbachia和cif基因的同源物。双重感染的男性诱导CI与未感染的女性，但不是女性单独感染CI引起沃尔巴克氏体。通过回交和随后的ddRAD SNPs分析，确定了一个影响MK的快速传播的显性核抑制遗传元件。拟高桥虫基因组这些发现突出了影响MK内共生体检测和种群动态的核和微生物组分的复杂性，以及内共生体与受其影响的宿主表型之间建立联系的挑战。沃尔巴克氏体细菌生活在许多昆虫的细胞中，对宿主有多种生殖作用。这项研究揭示了一种苍蝇物种的意外复杂性，即杀死雄性的沃尔巴克氏体菌株与导致同一宿主不相容的第二种菌株共存。
Bacteria that live inside the cells of insect hosts (endosymbionts) can alter the reproduction of their hosts, including the killing of male offspring (male killing, MK). MK has only been described in a few insects, but this may reflect challenges in detecting MK rather than its rarity. Here, we identify MK Wolbachia at a low frequency (around 4%) in natural populations of Drosophila pseudotakahashii. MK Wolbachia had a stable density and maternal transmission during laboratory culture, but the MK phenotype which manifested mainly at the larval stage was lost rapidly. MK Wolbachia occurred alongside a second Wolbachia strain expressing a different reproductive manipulation, cytoplasmic incompatibility (CI). A genomic analysis highlighted Wolbachia regions diverged between the 2 strains involving 17 genes, and homologs of the wmk and cif genes implicated in MK and CI were identified in the Wolbachia assembly. Doubly infected males induced CI with uninfected females but not females singly infected with CI-causing Wolbachia. A rapidly spreading dominant nuclear suppressor genetic element affecting MK was identified through backcrossing and subsequent analysis with ddRAD SNPs of the D. pseudotakahashii genome. These findings highlight the complexity of nuclear and microbial components affecting MK endosymbiont detection and dynamics in populations and the challenges of making connections between endosymbionts and the host phenotypes affected by them. Wolbachia bacteria live in the cells of many insects and have a variety of reproductive effects on their host. This study reveals unexpected complexity in a fly species, whereby a Wolbachia strain that kills males lives alongside a second strain that causes incompatibility in the same host.
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影响因子：
3.3
作者：
Cooper, Brandon S.;Vanderpool, Dan;Turelli, Michael
通讯作者：
Turelli, Michael
影响因子：
9.8
作者：
Hornett EA;Charlat S;Duplouy AM;Davies N;Roderick GK;Wedell N;Hurst GD
通讯作者：
Hurst GD
DOI：
10.1098/rspb.2021.2781
发表时间：
2022-04-13
影响因子：
4.7
作者：
Hornett, Emily A.;Kageyama, Daisuke;Hurst, Gregory D. D.
通讯作者：
Hurst, Gregory D. D.
影响因子：
1.9
作者：
HOFFMANN, AA
通讯作者：
HOFFMANN, AA
影响因子：
28.3
作者：
Gerth, Michael;Bleidorn, Christoph
通讯作者：
Bleidorn, Christoph