NMR assignments and solution conformation of the DNA.RNA hybrid duplex d(GTGAACTT).r(AAGUUCAC).
NMR assignments and solution conformation of the DNA.RNA hybrid duplex d(GTGAACTT).r(AAGUUCAC).
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DNA.RNA 杂合双链体 d(GTGAACTT).r(AAGUUCAC) 的 NMR 分配和溶液构象。
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Tom Brown
中科院分区：
文献类型：
--
作者：
Andrew N. Lane;S. Ebel;Tom Brown
Nearly complete assignments of the 1H-NMR spectrum of the DNA.RNA hybrid d(GTGAACTT).r(AAGUUCAC) have been obtained by two-dimensional methods. Three-bond coupling constants measured from cross-sections of two-dimensional NOE spectra and double quantum-filtered correlation spectra showed that the sugars in the DNA strand are predominantly in the S domain of puckers, whereas the ribofuranoses are mainly C3'-endo. Analysis of time-dependent NOE intensities from one- and two-dimensional experiments showed that the glycosidic torsion angles in the DNA strand are near -120 degrees, whereas those in the RNA strand are in the range- 140 degrees to -160 degrees. These nucleotide conformations correspond to those typically found in B-DNA and A-RNA, respectively. The circular dichroism of the duplex is similar to that of A-form RNA, consistent with a global A-like conformation. A large number of duplex structures was generated in which the nucleotides were fixed in the experimentally determined conformations. A subset of these structures was found that satisfied the internucleotide NOE intensities, backbone constraints and had acceptable Lennard-Jones energies. The base pairs in the duplex were found to have positive inclinations, a translation (Dx) of about 0.4 nm from the helix axis, and more than 10 bp/turn on average. This implies a helical structure in the A family of conformations.
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影响因子：
2.9
作者：
Miguel Salazar;J. Champoux;Brian R. Reid
通讯作者：
Miguel Salazar;J. Champoux;Brian R. Reid
影响因子：
2.9
作者：
SCHMITZ, U;ZON, G;JAMES, TL
通讯作者：
JAMES, TL
含有 GT、GG、GA、AC 和 GU 碱基对错配的双链寡脱氧核糖核苷酸十二聚体的脱氧核糖磷酸骨架构象扭曲对 31P NMR 谱的影响。
影响因子：
2.9
作者：
Roongta,VA;Jones,CR;Gorenstein,DG
通讯作者：
Gorenstein,DG
影响因子：
56.9
作者：
YANG, W;HENDRICKSON, WA;SATOW, Y
通讯作者：
SATOW, Y
含有共有 pribnow 启动子序列的合成 DNA-RNA 杂合十二聚体的高分辨率 NMR 研究：d(CGTTATAATGCG).r(CGCAUUAUAACG)。
影响因子：
2.9
作者：
Chou,SH;Flynn,P;Reid,B
通讯作者：
Reid,B