Effect of distortions in the deoxyribose phosphate backbone conformation of duplex oligodeoxyribonucleotide dodecamers containing GT, GG, GA, AC, and GU base-pair mismatches on 31P NMR spectra.
Effect of distortions in the deoxyribose phosphate backbone conformation of duplex oligodeoxyribonucleotide dodecamers containing GT, GG, GA, AC, and GU base-pair mismatches on 31P NMR spectra.
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含有 GT、GG、GA、AC 和 GU 碱基对错配的双链寡脱氧核糖核苷酸十二聚体的脱氧核糖磷酸骨架构象扭曲对 31P NMR 谱的影响。
DOI：
10.1021/bi00474a005
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Gorenstein,DG
中科院分区：
文献类型：
--
作者：
Roongta,VA;Jones,CR;Gorenstein,DG
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907 Received December 12, 1989; Revised Manuscript Received February 2, 1990 abstract: We have previously suggested that variations inthe 31P chemical shifts of individual phosphates in duplex oligonucleotides are attributable to torsional angle changes in the deoxyribose phosphate backbone. This hypothesis is now directly supported by analysis of the* H/31P two-dimensional/-resolved spectra of a number of mismatch dodecamer oligonucleotide duplexes including the following sequences: d-(CGTGAATTCGCG), d (CGUGAATTCGCG), d (CGGGAATTCGCG), d (CGAGAATTCGCG), and d (CGCGAATTCACG). The 31P NMR signals of the dodecamer mismatch duplexes were assigned by 2D'H/31P pure absorption phase constant time (PAC) heteronuclear correlation spectra. From the assigned H3'and H4'signals, the 31P signals of the base-pair mismatch dodecamers were identified./H3, _P coupling constants for each of the phosphates of the dodecamers were obtained from ‘H/31P/-resolved selective proton flip 2D spectra. By use of a modified Karplus relationship, the C4'-C3'-03'-P torsional angles (e) were obtained./H3/_P coupling constants were measured for many of the oligonucleotides as a function of temperature. There exists a good linear correlation between 31P chemical shifts and the e torsional angle. This correlation can be further extended to the C3'-03'-P-05'torsional angle (f) by using a linear relationship between e and f obtained from crystal structure studies. The 31P chemical shifts follow the general observation that the more internally the phosphate is located within the oligonucleotide sequence, the more upfield the 31P resonance occurs. In addition, 31P chemical shifts showsequence-andsite-specific variations. Analysis of the backbone torsional angle variations from the coupling constant analysis has provided additional information regarding the origin of these variations in 31P chemical shifts.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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