Flow Cytometry-Based Determination of Ploidy from Dried Leaf Specimens in Genomically Complex Collections of the Tropical Forage Grass Urochloa s. l.
Flow Cytometry-Based Determination of Ploidy from Dried Leaf Specimens in Genomically Complex Collections of the Tropical Forage Grass Urochloa s. l.
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DOI：
10.3390/genes12070957
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发表时间：
2021-06-23
期刊：
影响因子：
3.5
通讯作者：
Heslop-Harrison PJS
中科院分区：
文献类型：
--
作者：
Tomaszewska P;Pellny TK;Hernández LM;Mitchell RAC;Castiblanco V;de Vega JJ;Schwarzacher T;Heslop-Harrison PJS
尾序草属（包括臂形草属、大盖龙属和一些黍属）热带草原产于非洲，经过选育，现已在世界各地特别是南美洲种植，作为重要的牧草，具有进一步可持续改善和保护草原的巨大潜力。我们的目的是开发一种优化的方法来确定种质收集的热带牧草组使用干燥的叶片材料，包括方法收集，干燥和保存植物样品流式细胞仪分析的倍性。我们的方法能够稳健地鉴定倍性水平（G 0/G1峰的变异系数，CV，通常<5%）。利用我们定义的标准方案，对来自国际遗传资源库的348份牧草种质（倍性范围从2x到9 x）的倍性进行了测定，这些种质表现出基本染色体数目和生殖模式（无融合生殖和有性生殖）的变异。两个主要的Urophila无配子复合体被用于CIAT和EMBRAPA当前的育种计划：'brizantha'和'humidicola'无配子复合体是可变的，具有多倍性水平。一些美国人。brizantha种质具有奇数倍性水平（5x），相邻细胞型之间峰位荧光值的相对差异减小，因此需要对该物种进行更精确的检测。倍性测定。humidicola为非整倍体。
Urochloa (including Brachiaria, Megathyrus and some Panicum) tropical grasses are native to Africa and are now, after selection and breeding, planted worldwide, particularly in South America, as important forages with huge potential for further sustainable improvement and conservation of grasslands. We aimed to develop an optimized approach to determine ploidy of germplasm collection of this tropical forage grass group using dried leaf material, including approaches to collect, dry and preserve plant samples for flow cytometry analysis. Our methods enable robust identification of ploidy levels (coefficient of variation of G0/G1 peaks, CV, typically <5%). Ploidy of some 348 forage grass accessions (ploidy range from 2x to 9x), from international genetic resource collections, showing variation in basic chromosome numbers and reproduction modes (apomixis and sexual), were determined using our defined standard protocol. Two major Urochloa agamic complexes are used in the current breeding programs at CIAT and EMBRAPA: the ’brizantha’ and ’humidicola’ agamic complexes are variable, with multiple ploidy levels. Some U. brizantha accessions have odd level of ploidy (5x), and the relative differences in fluorescence values of the peak positions between adjacent cytotypes is reduced, thus more precise examination of this species is required. Ploidy measurement of U. humidicola revealed aneuploidy.
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影响因子：
4.2
作者：
Francis, Dennis;Davies, M. Stuart;Barlow, Peter W.
通讯作者：
Barlow, Peter W.
DOI：
10.1590/s1806-66902014000100008
发表时间：
2014-03-01
期刊：
Revista Ciência Agronômica
影响因子：
--
作者：
Timbó, Ana Luiza de Oliveira;Pereira, Roselaine Cristina;Davide, Lisete Chamma
通讯作者：
Davide, Lisete Chamma
影响因子：
2.6
作者：
da Rocha, Mara Jane;Chiavegatto, Raquel Bezerra;Techio, Vania Helena
通讯作者：
Techio, Vania Helena
影响因子：
5.6
作者：
Capstaff NM;Miller AJ
通讯作者：
Miller AJ
影响因子：
4.2
作者：
Dolezel, J;Bartos, J
通讯作者：
Bartos, J