Genome-Wide Identification and Analysis of Lipases in Fig Wasps (Chalcidoidea, Hymenoptera).
Genome-Wide Identification and Analysis of Lipases in Fig Wasps (Chalcidoidea, Hymenoptera).
复制标题
作者：
脂肪酶是参与脂肪代谢的一大类酶。脂质在昆虫的生长和对环境刺激的反应中发挥着不同的作用。无花果黄蜂是在无花果树花序中发育的无花果总科的一个多系组合。根据是否能授粉，可将其分为传粉性无花果小蜂(PFW)和非传粉性无花果小蜂(NPFW)。在这项研究中，我们使用生物信息学工具对12个无花果黄蜂基因组中的脂肪酶进行了全基因组筛选，其中包括7个PFW和5个NPFW。共鉴定出481个脂肪酶基因，其中中性脂肪酶和酸性脂肪酶是数量最多的家族。NPFW的脂肪酶显著多于PFW。串联复制是基因家族扩大的原因。系统进化分析表明，脂肪酶基因是保守的。这项研究为了解无花果和无花果黄蜂之间的专性互惠关系提供了昆虫代谢的证据。我们的结果将有助于理解脂肪酶蛋白如何有助于区分PFW和NPFW的生活史的分子机制。脂肪酶是参与脂肪代谢的主要酶类。然而，对昆虫脂肪酶特性的研究却很少。在这里，我们筛选了最近测序的12种无花果小蜂的基因组，其中包括7种传粉无花果小蜂(PFWs)和5种非授粉无花果小蜂(NPFWs)，分别属于六个主要的脂肪酶基因家族。共鉴定了481个脂肪酶基因，其中中性脂肪酶和酸性脂肪酶是最多的两个家族。串联复制是基因家族扩大的原因。NPFW的脂肪酶显著多于PFW。在PFW分支中出现了一个显著的基因家族收缩。NPFW和PFW之间脂肪酶的差异可能是它们在生活史和喂养制度上存在差异的原因之一。系统发育分析表明，各种榕树小蜂的脂肪酶基因在各分支中的分布基本一致，表明脂肪酶基因是保守的。基因结构在各支系内相似，但在支系间有所不同。中性脂肪酶和酸性脂肪酶多为信号肽，定位于细胞外。预测了脂肪酶参与的途径。这项全基因组研究对12种膜翅目昆虫的脂肪酶基因家族进行了系统的分析，并为理解脂肪酶的潜在功能提供了进一步的见解。
Lipases are a large family of enzymes involved in lipid metabolism. Lipids play diverse roles in insect growth and responses to environmental stimuli. Fig wasps are a polyphyletic assemblage of Chalcidoidea that develop in the inflorescences of fig trees. Based on whether they can pollinate, they are separated into pollinator fig wasp (PFW) and non-pollinating fig wasp (NPFW). In this study, we conducted a genome-wide screening of lipases in the 12 fig wasp genomes using bioinformatics tools, including seven PFWs and five NPFWs. In total, 481 lipase genes were identified with the neutral and acid lipases as the most numerous families. NPFWs had significantly more lipases than PFWs. Tandem duplication accounted for the expansion of the gene family. Phylogenetic analysis indicated that the lipase genes were conserved. This study provided evidence of insect metabolism to understand the obligate mutualism between figs and fig wasps. Our results will facilitate the understanding of the molecular mechanism of how lipase proteins contribute to the distinctions of life histories between PFWs and NPFWs. Lipases are the main enzymes involved in lipid metabolism. However, the characteristics of lipases in insects were scarcely investigated. Here, we screened the recently sequenced genomes of 12 fig wasp species consisting of seven pollinator fig wasps (PFWs) and five non-pollinating fig wasps (NPFWs) for the six major lipase gene families. In total, 481 lipase genes were identified, and the two most numerous families were the neutral and acid lipases. Tandem duplication accounted for the expansion of the gene family. NPFWs had significantly more lipases than PFWs. A significant gene family contraction occurred in the clade of PFWs. The difference of lipases between NPFWs and PFWs might contribute to their distinction in life histories and feeding regimes. Phylogenetic analysis showed that the lipase genes of each fig wasp species was almost equally distributed in each clade, indicating that the lipase genes were conserved. The gene structures were similar within each clade, while they were different among clades. Most of the neutral and acid lipases were signal peptides and located extracellularly. The pathways of lipases involved were predicted. This genome-wide study provides a systematic analysis of lipase gene families in 12 hymenopteran insects and further insights towards understanding the potential functions of lipases.
登录
查看更多内容
DOI：
10.1016/s0304-4157(98)00016-1
发表时间：
1998-11-10
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON BIOMEMBRANES
影响因子：
--
作者：
Carrière, F;Withers-Martinez, C;Verger, R
通讯作者：
Verger, R
影响因子：
29.4
作者：
Carrière, F;Renou, C;Verger, R
通讯作者：
Verger, R
影响因子：
56.9
作者：
HOLM, C;KIRCHGESSNER, TG;SCHOTZ, MC
通讯作者：
SCHOTZ, MC
影响因子：
10.7
作者：
Achaz, G;Coissac, E;Netter, P
通讯作者：
Netter, P
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS