FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells.
FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells.
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DOI：
10.1038/srep06024
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发表时间：
2014-08-12
影响因子：
4.6
通讯作者：
Chien S
中科院分区：
文献类型：
--
作者：
Hu YL;Lu S;Szeto KW;Sun J;Wang Y;Lasheras JC;Chien S
细胞迁移需要许多蛋白质的精细时空整合，这些蛋白质调节驱动细胞运动的基本过程。粘着斑（focal adhesion，FA）动力学是一个连续的过程，涉及FA与肌动蛋白细胞骨架之间的协调作用，这对细胞迁移至关重要。我们研究了粘着斑激酶（FAK）和桩蛋白在活的内皮细胞突起的FAs的动态之间的时空关系。同时双色成像显示FAK首先在FA组装，然后是桩蛋白募集到FA。通过跟踪和定量迁移细胞中的FAK和桩蛋白，标准化的FAK/桩蛋白荧光强度（FI）比在细胞前部> 1（1.4倍），在细胞中心> 1，并且在细胞后部<1。细胞前端FAK FI显著高于桩蛋白FI，表明FAK-FA在细胞前端的组装先于桩蛋白。为了确定FAK和桩蛋白在新生FA处组装之间的时间差，通过图像分析和时间相关性对含有FAK和桩蛋白两者的FA进行定量。结果表明，FAK比桩蛋白更早地聚集在细胞前端突起的新生FA上。
Cell migration requires the fine spatiotemporal integration of many proteins that regulate the fundamental processes that drive cell movement. Focal adhesion (FA) dynamics is a continuous process involving coordination between FA and actin cytoskeleton, which is essential for cell migration. We studied the spatiotemporal relationship between the dynamics of focal adhesion kinase (FAK) and paxillin at FAs in the protrusion of living endothelial cells. Concurrent dual-color imaging showed that FAK was assembled at FA first, which was followed by paxillin recruitment to the FA. By tracking and quantifying FAK and paxillin in migrating cells, the normalized FAK/Paxillin fluorescence intensity (FI) ratio is > 1 (≈4 fold) at cell front, ≈1 at cell center, and < 1 at cell rear. The significantly higher FAK FI than paxillin FI at cell front indicates that the assembly of FAK-FAs occurs ahead of paxillin at cell front. To determine the time difference between the assemblies of FAK and paxillin at nascent FAs, FAs containing both FAK and paxillin were quantified by image analysis and time correlation. The results show that FAK assembles at the nascent FAs earlier than paxillin in the protrusions at cell front.
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DOI：
10.1038/nrm2957
发表时间：
2010-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4
作者：
Zaidel-Bar, R;Ballestrem, C;Geiger, B
通讯作者：
Geiger, B
DOI：
10.1083/jcb.200708194
发表时间：
2008-01-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lim Y;Lim ST;Tomar A;Gardel M;Bernard-Trifilo JA;Chen XL;Uryu SA;Canete-Soler R;Zhai J;Lin H;Schlaepfer WW;Nalbant P;Bokoch G;Ilic D;Waterman-Storer C;Schlaepfer DD
通讯作者：
Schlaepfer DD
影响因子：
3.4
作者：
Digman, Michelle A.;Brown, Claire M.;Gratton, Enrico
通讯作者：
Gratton, Enrico
影响因子：
4
作者：
Zaidel-Bar, Ronen;Milo, Ron;Geiger, Benjamin
通讯作者：
Geiger, Benjamin