Growth factor‐induced proliferation of human fibroblasts in serum‐free culture depends on cell density and extracellular calcium concentration
Growth factor‐induced proliferation of human fibroblasts in serum‐free culture depends on cell density and extracellular calcium concentration
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无血清培养中生长因子诱导的人成纤维细胞增殖取决于细胞密度和细胞外钙浓度
DOI：
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发表时间：
1984
影响因子：
5.6
通讯作者：
B. Westermark
中科院分区：
文献类型：
--
作者：
C. Betsholtz;B. Westermark
在没有血清或血清衍生蛋白的情况下，人新生儿皮肤成纤维细胞稀疏地镀在MCDB 105中穿越了一个完整的细胞周期。3H‐胸腺嘧啶标记指数和钯岛克隆生长显示，添加纯PDGF并没有显著增加进入S期的时间。在亚生理Ca2+浓度或钙调素抑制剂W7的存在下，生长因子缺失时增殖停止，PDGF成为有丝分裂细胞。相比之下，PDGF在生理Ca2+浓度下刺激融合成纤维细胞培养。这也是稀疏的成人皮肤成纤维细胞培养的情况，而胎儿菌株在缺乏PDGF的情况下进入S，即使在低细胞外Ca2+浓度下也是如此。在所有实验中，EGF和PDGF的结果相似。这表明这两种生长因子在细胞周期中具有相似的作用。然而，PDGF和EGF的作用机制存在差异，这一事实表明，当单个生长因子的最大生长反应受到亚生理性细胞外Ca2+浓度的限制时，PDGF和EGF在最佳浓度下是相加的。
Human neonatal skin fibroblasts plated sparsely in MCDB 105 traversed a complete cell cycle in the absense of serum or serum‐derived proteins. Addition of pure PDGF did not significantly increase entrance into S phase as revealed by 3H‐thymidine labeling index or clonal growth on palladium islands. In subphysiologic Ca2+ concentrations or in the presence of a calmodulin inhibitor, W7, proliferation in the absence of growth factors ceased and PDGF became mitogenic. In contrast, confluent fibroblast cultures were stimulated by PDGF in physiologic Ca2+ concentrations. This was also the case with sparse adult skin fibroblast cultures while a fetal strain entered S in the absence of PDGF even in low extracellular Ca2+ concentrations. EGF gave similar results as PDGF in all experiments performed. This proposes a similar role for the two growth factors in the cell cycle. However, a difference in the mechanisms of action of PDGF and EGF is indicated by the fact that PDGF and EGF were additive at optimal concentrations when maximal growth response by a single growth factor was restricted by a subphysiologic extracellular Ca2+ concentration.
DOI：
10.1073/pnas.76.4.1809
发表时间：
1979-01-01
影响因子：
11.1
作者：
ANTONIADES, HN;SCHER, CD;STILES, CD
通讯作者：
STILES, CD
培养的人成纤维细胞产生免疫反应性生长调节素的激素控制。
DOI：
10.1172/jci110001
发表时间：
1981
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Clemmons,DR;Underwood,LE;VanWyk,JJ
通讯作者：
VanWyk,JJ