High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon.
High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon.
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DOI：
10.1186/s13071-018-2787-7
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发表时间：
2018-03-20
影响因子：
3.2
通讯作者：
Lacerda MVG
中科院分区：
文献类型：
--
作者：
Almeida ACG;Kuehn A;Castro AJM;Vitor-Silva S;Figueiredo EFG;Brasil LW;Brito MAM;Sampaio VS;Bassat Q;Felger I;Tadei WP;Monteiro WM;Mueller I;Lacerda MVG
在拉丁美洲进行的基于人群的研究表明，无症状和亚显微镜下疟疾感染的比例很高。考虑到旨在区域消除的努力，重要的是调查这一无症状宿主在城郊地区疟疾传播中的作用。本研究旨在估计巴西亚马逊地区有症状和无症状感染的疟原虫和配子细胞负荷的流行情况。对巴西西亚马逊州马瑙斯城郊地区的所有居民进行了两次横断面住户调查。采用光镜（LM）和qPCR检测疟疾寄生虫。采用LM法和RT-qPCR法检测疟原虫的性分期。两次调查共纳入4083名参与者。CS1区间日疟原虫qPCR阳性率为4.3% (86/2010)，LM阳性率为1.6%（32/2010）。50%（43/86）的间日疟原虫感染者（qPCR）携带间日疟原虫配子细胞。在CS2中，3.4%（70/2073）的参与者有qpcr检测到间日疟原虫感染，其中42.9%（30/70）的感染者配子细胞阳性。间日疟原虫密度与配子体携带量相关（P < 0.001）。67%的间日疟感染者和53.4%的间日疟配子细胞携带者无症状。本研究证实了研究地区无症状和亚显微间日疟原虫感染的相当大比例。大多数无症状个体携带配子体，表现为低无性寄生虫血症。这一水库积极促进了巴西亚马逊地区的疟疾传播，强调有必要实施更有效的控制和消除战略。本文的在线版本（10.1186/s13071-018-2787-7）包含补充材料，授权用户可使用。
Population-based studies conducted in Latin America have shown a high proportion of asymptomatic and submicroscopic malarial infections. Considering efforts aiming at regional elimination, it is important to investigate the role of this asymptomatic reservoir in malaria transmission in peri-urban areas. This study aimed to estimate the prevalence of Plasmodium spp. and gametocyte burden on symptomatic and asymptomatic infections in the Brazilian Amazon. Two cross-sectional household surveys (CS) were conducted including all inhabitants in a peri-urban area of Manaus, western Amazonas State, Brazil. Malaria parasites were detected by light microscopy (LM) and qPCR. Sexual stages of Plasmodium spp. were detected by LM and RT-qPCR. A total of 4083 participants were enrolled during the two surveys. In CS1, the prevalence of Plasmodium vivax infections was 4.3% (86/2010) by qPCR and 1.6% (32/2010) by LM. Fifty percent (43/86) of P. vivax infected individuals (qPCR) carried P. vivax gametocytes. In CS2, 3.4% (70/2073) of participants had qPCR-detectable P. vivax infections, of which 42.9% (30/70) of infections were gametocyte positive. The P. vivax parasite density was associated with gametocyte carriage (P < 0.001). Sixty-seven percent of P. vivax infected individuals and 53.4% of P. vivax gametocyte carriers were asymptomatic. This study confirms a substantial proportion of asymptomatic and submicroscopic P. vivax infections in the study area. Most asymptomatic individuals carried gametocytes and presented low asexual parasitemia. This reservoir actively contributes to malaria transmission in the Brazilian Amazon, underscoring a need to implement more efficient control and elimination strategies. The online version of this article (10.1186/s13071-018-2787-7) contains supplementary material, which is available to authorized users.
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亚显微间日疟原虫感染的系统评价。
影响因子：
3
作者：
Moreira CM;Abo-Shehada M;Price RN;Drakeley CJ
通讯作者：
Drakeley CJ
DOI：
10.1086/597414
发表时间：
2009-04-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Putaporntip C;Hongsrimuang T;Seethamchai S;Kobasa T;Limkittikul K;Cui L;Jongwutiwes S
通讯作者：
Jongwutiwes S
影响因子：
9.4
作者：
Perandin, F;Manca, N;Chezzi, C
通讯作者：
Chezzi, C
影响因子：
2.7
作者：
da Silva-Nunes, Monica;Moreno, Marta;Ferreira, Marcelo U.
通讯作者：
Ferreira, Marcelo U.
影响因子：
3.8
作者：
Barbosa, Susana;Gozze, Amanda B.;Ferreira, Marcelo U.
通讯作者：
Ferreira, Marcelo U.