Differential prevalence of Plasmodium infections and cryptic Plasmodium knowlesi malaria in humans in Thailand.
Differential prevalence of Plasmodium infections and cryptic Plasmodium knowlesi malaria in humans in Thailand.
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DOI：
10.1086/597414
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发表时间：
2009-04-15
期刊：
影响因子：
--
通讯作者：
Jongwutiwes S
中科院分区：
文献类型：
--
作者：
Putaporntip C;Hongsrimuang T;Seethamchai S;Kobasa T;Limkittikul K;Cui L;Jongwutiwes S
最近在泰国发现了一例人感染诺氏疟原虫的病例。为了调查这种疟疾的流行情况，进行了一项基于分子的调查。通过显微镜和巢式PCR检测了1，874例患者的血液样本中的疟原虫。通过对裂殖子表面蛋白-1（msp-1）基因进行测序来表征诺氏疟原虫。其中恶性疟原虫、间日疟原虫、三日疟原虫、卵形疟原虫和诺氏疟原虫分别占43.52%、68.08%、1.37%、1.03%和0.57%。与缅甸接壤的西北部和西南部地区（23-24%）的混合物种感染比东部和南部地区（3-5%）更常见。在南部和西南部地区，混合感染在旱季的患病率显著高于雨季（p<0.003）。10例患者中发现诺氏疟原虫，主要来自南部和西南部地区。其中9例同时感染了恶性疟原虫或间日疟原虫。大多数诺氏疟原虫泰国分离株与来自猕猴的疟疾比来自沙捞越患者的分离株更相关。来自同一流行区的诺氏疟原虫msp-1基因序列存在差异，并具有新的序列，表明诺氏疟原虫感染人类的遗传多态性。这项调查强调了诺氏疟原虫在泰国的广泛分布，尽管患病率较低，而且大多数情况下是隐性感染。
A case of human infection with Plasmodium knowlesi has been recently discovered in Thailand. To investigate the prevalence of this malaria species, a molecular-based survey was performed. Blood samples from 1,874 patients were determined for Plasmodium species by microscopy and nested PCR. Characterization of P. knowlesi was done by sequencing the merozoite surface protein-1 (msp-1) gene. Of all species identified, P.falciparum, P.vivax, P.malariae, P.ovale and P.knowlesi contributed 43.52, 68.08, 1.37, 1.03 and 0.57%, respectively. Mixed species infections were more common in northwestern and southwestern regions bordering Myanmar (23-24%) than in eastern and southern areas (3-5%). In southern and southwestern regions, mixed species infections had significantly higher prevalence in dry than in rainy seasons (p<0.003). P.knowlesi was found in 10 patients, mostly from southern and southwestern areas. Nine of these were co-infected with either P.falciparum or P.vivax. Most of the P.knowlesi Thai isolates were more related with malaria from macaques than isolates from Sarawak patients. The msp-1 sequences of isolates from the same endemic area differed and possessed novel sequences, indicating genetic polymorphism in P.knowlesi infecting humans. This survey highlighted the widespread distribution of P.knowlesi in Thailand, albeit at low prevalence and mostly occurring as cryptic infections.
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影响因子：
--
作者：
Looareesuwan, S;Buchachart, K;Walsh, DS
通讯作者：
Walsh, DS
DOI：
10.4269/ajtmh.2003.68.613
发表时间：
2003-05-01
影响因子：
3.3
作者：
Cui, LW;Mascorro, CN;Sattabongkot, J
通讯作者：
Sattabongkot, J
影响因子：
3.5
作者：
Putaporntip, Chaturong;Jongwutiwes, Somchai;Hughes, Austin L.
通讯作者：
Hughes, Austin L.
影响因子：
11.8
作者：
Jongwutiwes S;Putaporntip C;Iwasaki T;Sata T;Kanbara H
通讯作者：
Kanbara H
DOI：
10.1016/0035-9203(95)90525-1
发表时间：
1995-05-01
影响因子：
2.2
作者：
SOMBOON, P;LINES, J;KHAMBOONRUANG, C
通讯作者：
KHAMBOONRUANG, C