Cell wall components and pectin esterification levels as markers of proliferation and differentiation events during pollen development and pollen embryogenesis in Capsicum annuum L.
Cell wall components and pectin esterification levels as markers of proliferation and differentiation events during pollen development and pollen embryogenesis in Capsicum annuum L.
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DOI：
10.1093/jxb/erp392
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发表时间：
2010-02
影响因子：
6.9
通讯作者：
Testillano PS
中科院分区：
文献类型：
--
作者：
Bárány I;Fadón B;Risueño MC;Testillano PS
植物细胞壁及其聚合物在植物发育过程中受到调控，但其分子组分的具体作用以及壁变化在不同细胞类型和过程中的功能意义尚不清楚。在这项工作中，在两个发展计划，配子体花粉发育，这是一个分化过程，和应力诱导的花粉胚胎发生，其中涉及增殖，然后分化过程中的不同细胞壁成分的分布进行原位分析。细胞壁聚合物的变化进行了比较与植物细胞增殖和分化的系统，根顶端分生组织。在合子胚胎发生的第一阶段也进行了分析。识别主要细胞壁聚合物，木葡聚糖（XG）和鼠李半乳糖醛酸聚糖II（RGII）果胶结构域的特异性抗体，以及针对高和低甲基酯化果胶的抗体用于斑点印迹和免疫定位与光学和电子显微镜。结果表明，这些分子复合物的分布模式的差异，以及酯化和非酯化果胶的比例在两个花粉发育途径。高度酯化的果胶是增殖的特征，而高水平的非酯化的果胶，XG和RGII是丰富的分化细胞壁。合子胚中的分布模式与花粉胚相似。报道的细胞壁变化是花粉发育和胚胎发生过程中发生的增殖和分化事件的特征。
Plant cell walls and their polymers are regulated during plant development, but the specific roles of their molecular components are still unclear, as well as the functional meaning of wall changes in different cell types and processes. In this work the in situ analysis of the distribution of different cell wall components was performed during two developmental programmes, gametophytic pollen development, which is a differentiation process, and stress-induced pollen embryogenesis, which involves proliferation followed by differentiation processes. The changes in cell wall polymers were compared with a system of plant cell proliferation and differentiation, the root apical meristem. The analysis was also carried out during the first stages of zygotic embryogenesis. Specific antibodies recognizing the major cell wall polymers, xyloglucan (XG) and the rhamnogalacturonan II (RGII) pectin domain, and antibodies against high- and low-methyl-esterified pectins were used for both dot-blot and immunolocalization with light and electron microscopy. The results showed differences in the distribution pattern of these molecular complexes, as well as in the proportion of esterified and non-esterified pectins in the two pollen developmental pathways. Highly esterified pectins were characteristics of proliferation, whereas high levels of the non-esterified pectins, XG and RGII were abundant in walls of differentiating cells. Distribution patterns similar to those of pollen embryos were found in zygotic embryos. The wall changes reported are characteristic of proliferation and differentiation events as markers of these processes that take place during pollen development and embryogenesis.
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影响因子：
2.7
作者：
Bárány, I;González-Melendi, P;Testillano, PS
通讯作者：
Testillano, PS
影响因子：
3
作者：
Coronado, MJ;González-Melendi, P;Risueño, MC
通讯作者：
Risueño, MC
影响因子：
2.9
作者：
GONZALEZMELENDI, P;TESTILLANO, PS;RISUENO, MC
通讯作者：
RISUENO, MC
影响因子：
4.3
作者：
Guglielmino, N;Liberman, M;Goldberg, R
通讯作者：
Goldberg, R
影响因子：
7.4
作者：
Baluska, F;Hlavacka, A;Volkmann, D
通讯作者：
Volkmann, D