Triplet repeat-containing ribosomal protein L14 gene in immortalized human endothelial cell line (t-HUE4).
Triplet repeat-containing ribosomal protein L14 gene in immortalized human endothelial cell line (t-HUE4).
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永生化人内皮细胞系 (t-HUE4) 中含有三联体重复的核糖体蛋白 L14 基因。
DOI：
10.1006/bbrc.1998.8125
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发表时间：
1998
影响因子：
3.1
通讯作者：
Y. Mitsui
中科院分区：
文献类型：
--
作者：
M. Tanaka;T. Tanaka;M. Harata;T. Suzuki;Y. Mitsui
从人永生内皮细胞系t-HUE 4中分离出编码人60 S核糖体亚基蛋白L14（hRL 14）的cDNA。该细胞系是通过在无血清和无蛋白质培养基中培养的一系列细胞系建立的，并且已经构建了定向cDNA文库，并筛选了在细胞增殖中调节蛋白质合成机制的基因。具有220个氨基酸开放阅读框的推定全长克隆;预测分子量为23.6 kDa。观察到hRL 14与大鼠RL 14（85%同一性）的显著同一性，除了COOH末端区域，但与迄今为止保存到数据库中的任何真核生物氨基酸序列都不相同。在hRL 14中观察到核糖体蛋白的典型特征，如在从细胞质运输到核仁所必需的核靶向序列、用于结合rRNA的bZIP样（碱性区域-亮氨酸拉链）元件和内部重复序列中所见;五肽QKA（A/S）X。转录物的COOH末端区域在核苷酸465至509处含有15个三联体重复（GCT;丙氨酸），这与大鼠RL 14相比显著扩大。然而，在正常人内皮细胞系和在t-HUE 4建立过程中建立的细胞系中，重复数均相同。通过北方印迹分析鉴定了约800个碱基的单一条带，无组织特异性。发现该GCT重复序列是编码序列中最长的不间断重复序列之一，与最高程度的多态性相关。
A cDNA encoding human 60S ribosomal subunit protein L14 (hRL14) was isolated from a human immortal endothelial cell line, t-HUE4. This cell line was established via a series of cell lines cultured in a serum-free and a protein-free medium, and a directional cDNA library has been constructed and screened in search for the genes modulating protein synthesis machinery in cell proliferation. A putative full-length clone with an open reading frame of 220 amino acids; predicted molecular weight of 23.6 kDa. A significant identity for hRL14 was observed with rat RL14 (85% identity), with exception of COOH-terminal region, but not with any eukaryote amino acid sequences so far deposited to database. The typical features of ribosomal proteins were observed in hRL14, as seen in nuclear targeting sequences necessary for the transport from cytoplasm to nucleolus, a bZIP like (basic region-leucine zipper) element for the binding to rRNA, and the internal repeat sequences; the pentapeptide QKA(A/S)X. The COOH-terminal region of the transcripts contained fifteen triplet repeats (GCT; alanine) at nucleotide 465 to 509, which is significantly expanded compared to the rat RL14. However, the repeat number was all the same among the normal human endothelial cell line and the cell lines established in the course of t-HUE4 establishment. A single band with about 800 bases was identified by Northern blot analysis without tissue specificity. This GCT repeat was found to be one of the longest uninterrupted repeats in a coding sequence, which were associated with the highest degree of polymorphism.
大鼠核糖体蛋白L14的一级结构。
DOI：
10.1006/bbrc.1996.0761
发表时间：
1996
期刊：
Biochemical and biophysical research communications.
影响因子：
--
作者：
Chan,YL;Olvera,J;Wool,IG
通讯作者：
Wool,IG
大鼠核糖体蛋白S5的一级结构。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kuwano,Y;Olvera,J;Wool,IG
通讯作者：
Wool,IG
大鼠核糖体蛋白 L13a 中的亮氨酸拉链样基序和碱性区域亮氨酸拉链样元件。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chan,YL;Olvera,J;Glück,A;Wool,IG
通讯作者：
Wool,IG