A smartphone-based bacterial colony phenotyping instrument based on the reflective elastic light-scatter pattern
A smartphone-based bacterial colony phenotyping instrument based on the reflective elastic light-scatter pattern
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基于智能手机的基于反射弹性光散射图案的细菌菌落表型分析仪
DOI：
10.1117/12.2623007
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Bae, Euiwon
中科院分区：
文献类型：
--
作者：
Doh, Iyll-Joon;Dowden, Brianna;Patsekin, Valery;Rajwa, Bartek;Robinson, J. Paul;Bae, Euiwon
使用智能手机、绿色激光和投影屏幕材料，开发了一种基于菌落反射弹性光散射(ELS)图案的便携式细菌菌落分类工具。当来自激光的准直光束照射细菌菌落时，背向散射光子干涉并在屏幕材料上产生由菌落的独特形态决定的独特图案。位于屏幕后面的手机摄像头捕捉到了这种模式。收集的模式被用来提取不同生物之间的不同的散布相关特征，用于分类过程。与其他使用透射式ELS图案的工具不同，这种新型设备测量的是反射信号，因此这种ELS技术可以应用于生长在不透明介质上的生物体，如血液琼脂、巧克力琼脂，这些介质通常禁止光的传输和前向ELS图案的产生。将智能手机摄像头改装为成像设备后，该系统大大减少到了手掌大小的仪器。这使得它完全便携，便于携带。为了验证仪器的有效性，在不透明的琼脂上培养了两种不同的细菌，即大肠杆菌和无毒乳杆菌，并进行了测试。结果表明，在区分生物体方面，总体准确率超过90%。
A portable bacterial colony classification tool based on colonies’ reflective elastic light-scatter (ELS) patterns has been developed using a smartphone, a green laser, and a projection screen material. As the collimated beam from the laser illuminates the bacterial colony, backscattered photons interfere and generate a unique pattern on the screen material determined by the unique morphology of the colony. The phone camera, which is located behind the screen, captures the pattern. The collected patterns are utilized to extract the distinctive scatter-related features across different organisms for the classification process. Unlike other tools that use transmitted ELS patterns, the novel device measures the reflective signal, and therefore this ELS technique can be applied to organisms that are grown on opaque media such as blood agar, chocolate agar, which normally prohibits the transmission of the light and generation of forward ELS patterns. The adaptation of the smartphone camera as an imaging device dramatically reduced the system to a palm-size instrument. This made it wholly portable and easy to carry. For validation of the instrument, two different bacteria species, E. coli and L. innocua were grown on opaque agar media and tested. The results showed over 90% of overall accuracy in differentiating the organisms.
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影响因子：
5.3
作者：
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通讯作者：
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影响因子：
5
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
0.8
作者：
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通讯作者：
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影响因子：
3.7
作者：
Tang Y;Kim H;Singh AK;Aroonnual A;Bae E;Rajwa B;Fratamico PM;Bhunia AK
通讯作者：
Bhunia AK