Cytokines, polarity proteins, and endosomal protein trafficking and signaling-the sertoli cell blood-testis barrier system in vitro as a study model.
Cytokines, polarity proteins, and endosomal protein trafficking and signaling-the sertoli cell blood-testis barrier system in vitro as a study model.
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第十章 - 细胞因子、极性蛋白和内体蛋白运输和信号转导——支持细胞血液——体外睾丸屏障系统作为研究模型
DOI：
10.1016/b978-0-12-397926-1.00010-x
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发表时间：
2014
影响因子：
--
通讯作者：
Cheng, C. Yan
中科院分区：
文献类型：
--
作者：
Xiao, Xiang;Wong, Elissa W. P.;Lie, Pearl P. Y.;Mruk, Dolores D.;Wong, Chris K. C.;Cheng, C. Yan
内体信号转导是调节哺乳动物细胞或上皮细胞中信号转导功能的最重要的细胞事件之一，其响应于环境的变化，例如在生长和发育期间由细胞因子、毒物、热、离子介导的刺激的存在，以及其他细胞过程例如胞质分裂和精子发生。最近的研究表明，蛋白质内吞作用-内体信号传导的初始步骤-涉及极性蛋白的参与，例如分配缺陷蛋白6（Par 6）、Cdc 42和14-3-3（也称为Par 5），其进而受细胞因子（例如，TGF-β2、TGF-β3）和睾酮在哺乳动物睾丸支持细胞血睾屏障（BTB）上的作用。在这篇简短的方法论文中，我们提供了一个评估蛋白质内吞作用的详细方案，这是支持细胞BTB内体信号传导的初始步骤，也是最关键的步骤。这种生化内吞测定总结了我们过去十年的经验，这可能会与双标记免疫荧光分析一起进行，以评估蛋白质内吞作用。虽然我们正在使用体外支持细胞系统，模拟体内BTB，这种方法应该适用于几乎所有的哺乳动物细胞。
Endosomal signaling is emerging as one of the most important cellular events that regulate signaling function in mammalian cells or an epithelium in response to changes in environment such as the presence of stimuli mediated by cytokines, toxicants, heat, ions during growth and development, and other cellular processes such as cytokinesis and spermatogenesis. Recent studies have shown that protein endocytosis—the initial step of endosomal signaling—involves the participation of polarity proteins, such as partitioning defective protein 6 (Par6), Cdc42 and 14-3-3 (also known as Par5), which in turn is regulated by cytokines (e.g., TGF-β2, TGF-β3) and testosterone at the Sertoli cell blood–testis barrier (BTB) in the mammalian testis. In this short method paper, we provide a detailed protocol of assessing protein endocytosis, the initial and also the most critical step of endosomal signaling at the Sertoli cell BTB. This biochemical endocytosis assay summarizes our experience for the last decade, which should likely be performed in conjunction with the dual-labeled immunofluorescence analysis to assess protein endocytosis. While we are using a Sertoli cell in vitro system that mimics the BTB in vivo, this approach should be applicable to virtually all mammalian cells.
影响因子：
7.8
作者：
BODE, F;POCKRAND.H;BAUMANN, K;KINNE, R
通讯作者：
KINNE, R