Analysis of the pinocytic process in rat kidney. I. Isolation of pinocytic vesicles from rat kidney cortex.
Analysis of the pinocytic process in rat kidney. I. Isolation of pinocytic vesicles from rat kidney cortex.
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DOI：
10.1083/jcb.63.3.998
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发表时间：
1974-12
影响因子：
7.8
通讯作者：
KINNE, R
中科院分区：
文献类型：
--
作者：
BODE, F;POCKRAND.H;BAUMANN, K;KINNE, R
用辣根过氧化物酶（HRP）诱导大鼠肾脏的胞饮作用。以HRP为外源性标记物，通过差速离心和自由流电泳法对肾皮质匀浆后的胞饮囊泡制剂进行富集。通过酶分析和电子显微镜（包括特定染色程序）鉴定囊泡。与肾皮质匀浆相比，典型的刷状缘酶，如碱性磷酸酶、氨肽酶、5 '-核苷酸酶、溶酶体酸性磷酸酶和线粒体琥珀酸脱氢酶在囊泡组分中减少。葡萄糖-6-磷酸酶和Na+-K+-ATP酶在组分中仅轻微增加。这些结果表明，从大鼠肾皮质的胞饮小泡的制剂可以富集。它们具有不同于先前从肾组织中纯化的细胞器和膜的生化特征。
Pinocytosis was induced in rat kidney by exposure to horseradish peroxidase (HRP). Pinocytic vesicle preparations were enriched after homogenization of kidney cortex by differential centrifugation and free-flow electrophoresis with HRP as an exogenous marker. Vesicles were identified by enzymatic analysis and by electron microscopy, including specific staining procedures. Typical brush-border enzymes such as alkaline phosphatase, aminopeptidase, 5'-nucleotidase, lysosomal acid phosphatase, and mitochondrial succinic dehydrogenase were reduced in the vesicular fraction, compared to the kidney cortex homogenate. Glucose-6-phosphatase and Na+-K+-ATPase were only slightly increased in the fraction. These results indicate that preparations of pinocytic vesicles from rat kidney cortex can be enriched. They have biochemical characteristics that differ from those of the cell organelles and membranes previously purified from renal tissue.
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影响因子：
3.7
作者：
BRANDT, PW
通讯作者：
BRANDT, PW
影响因子：
3.2
作者：
GOLDFISCHER, S;NOVIKOFF, AB;ESSNER, E
通讯作者：
ESSNER, E
DOI：
10.1016/s0022-5320(66)80108-9
发表时间：
1966-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
MAUNSBACH, AB
通讯作者：
MAUNSBACH, AB
DOI：
10.1016/0005-2744(68)90176-9
发表时间：
1968-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
JORGENSEN, PL
通讯作者：
JORGENSEN, PL
影响因子：
3.6
作者：
JUST, M;HABERMANN, E
通讯作者：
HABERMANN, E