Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV-ve Oral Cancer Stem Cells.
Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV-ve Oral Cancer Stem Cells.
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DOI：
10.3389/fonc.2018.00412
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发表时间：
2018
影响因子：
4.7
通讯作者：
Das BC
中科院分区：
文献类型：
--
作者：
Bano N;Yadav M;Das BC
目的：探讨中药抗氧化剂姜黄素对HPV16+Ve/-Ve口腔癌干细胞增殖、口腔球形成及miRNA-21表达的影响。材料和方法：用流式细胞仪和干细胞标志物从HPV+Ve/HPV-Ve口腔癌细胞系中分离出口腔癌干细胞。采用四甲基偶氮唑盐比色法、球体比色法和定量逆转录聚合酶链式反应检测姜黄素的作用。结果：微摩尔浓度(0~50μM)的姜黄素对CSC的增殖、球囊形成和miRNA21的表达均有不同程度的抑制作用，且呈剂量依赖关系，对HPV阳性的CSCs的抑制作用最为明显。结论：姜黄素对口腔鳞癌细胞的增殖、干性和miRNA的抑制作用呈剂量依赖性，可能与其对口腔鳞状细胞癌的化疗增敏和抗癌作用有关。
Aim: To investigate the role of a herbal antioxidative compound curcumin on cell proliferation, orosphere formation and miRNA-21 expression in HPV16+ve/–ve oral cancer stem cells. Materials and Methods: Oral cancer stem cells were isolated from HPV+ve/HPV–ve oral cancer cell lines by FACS and stemness markers. MTT, spheroid assay and qRT-PCR were employed to examine the effects of curcumin. Results: Curcumin treatment in micromolar concentration (0–50 μM) demonstrated significant differential inhibition in CSC proliferation, orosphere formation and miRNA-21 expression in a dose dependent manner, the effect being highly pronounced in HPV positive CSCs. Conclusion: The strong and dose-dependent inhibitory effects of curcumin on cell proliferation, stemness and miRNA appear to be due to its chemosensitizing and anticancer effects on OSCC-CSCs.
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