Poor HIV control in HLA-B*27 and B*57/58 noncontrollers is associated with limited number of polyfunctional Gag p24-specific CD8+ T cells
Poor HIV control in HLA-B*27 and B*57/58 noncontrollers is associated with limited number of polyfunctional Gag p24-specific CD8+ T cells
复制标题
HLA-B*27 和 B*57/58 非控制者中 HIV 控制不佳与多功能 Gag p24 特异性 CD8 T 细胞数量有限有关
DOI：
--
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
P. Hansasuta
中科院分区：
文献类型：
--
作者：
N. Techakriengkrai;Y. Tansiri;P. Hansasuta
目的：分析HIV控制者的免疫应答，为我们研究宿主免疫应答在HIV控制中的作用提供了一个机会。设计：在本研究中，在两组临床上不同的HLA-B*27、HLA-B*57/58匹配个体、病毒血症控制者[血浆HIV载量（pVL）≤ 2000拷贝/ml]和非控制者（pVL >2000拷贝/ml）中评估HIV Gag p24特异性CD 8 + T细胞应答的功能质量，以确定其对自然HIV临床结果的影响。 方法：使用体外干扰素（IFN）-ELISpot试验筛选每个个体的HIV Gag p24特异性T细胞应答。细胞内细胞因子染色测定用于确定它们的功能质量（作为产生的细胞因子的数量）。结果：我们发现，与以往的研究相比，所有泰国志愿者与HLA-B*5801是统一的noncontrollers。病毒血症控制者的高功能性p24特异性CD 8 + T细胞数量显著多于非病毒血症控制者（P <0.05）。 在总p24和表位特异性水平上均观察到这种上级应答质量。此外，高功能质量Gag p24特异性CD 8 + T细胞的绝对数量与pVL显著负相关（r =-0.6984，P = 0.0006），并且与CD 4 + T细胞计数显著正相关（r = 0.5648，P = 0.0095）。 结论：我们得出结论，足够数量的高功能质量Gag p24特异性CD 8 + T细胞与天然HIV控制者状态密切相关。
Objectives:Analysis of immune response in HIV controllers, a unique group of infected individuals who are able to control HIV naturally, has provided us a chance to investigate the roles of host immune responses in HIV control. Design:In this study, the functional quality of HIV Gag p24-specific CD8+ T-cell responses was assessed in two groups of clinically distinct, HLA-B*27, HLA-B*57/58-matched individuals, viremic controllers [plasma HIV load (pVL) ⩽2000 copies/ml) and noncontrollers (pVL >2000 copies/ml) to determine its impacts on natural HIV clinical outcome. Methods:An ex-vivo interferon (IFN)-&ggr; ELISpot assay was used to screen for each individual's HIV Gag p24-specific T-cell responses. Intracellular cytokine staining assay was used to determine their functional quality (as number of cytokine being produced). Results:We found that, in contrast to previous studies, all Thai volunteers with HLA-B*5801 were uniformly noncontrollers. Viremic controllers were observed with a significantly larger number of high functional quality p24-specific CD8+ T cells than noncontrollers (P < 0.05). This superior quality of responses was observed at both total p24 and epitope-specific level. Moreover, the absolute number of high functional quality Gag p24-specific CD8+ T cells was significantly in a negative correlation with pVL (r = −0.6984, P = 0.0006) and also in a positive correlation with CD4+ T-cell count (r = 0.5648, P = 0.0095). Conclusion:We concluded that an adequate number of high functional quality Gag p24-specific CD8+ T cells is strongly associated with a natural HIV controller status.
登录
查看更多内容
影响因子：
20.3
作者：
Betts, Michael R.;Nason, Martha C.;Koup, Richard A.
通讯作者：
Koup, Richard A.
DOI：
10.1073/pnas.050567397
发表时间：
2000-03-14
影响因子：
11.1
作者：
Migueles, SA;Sabbaghian, MS;Connors, M
通讯作者：
Connors, M
影响因子：
20.3
作者：
Almeida, Jorge R.;Sauce, Delphine;Appay, Victor
通讯作者：
Appay, Victor
影响因子：
56.9
作者：
Schmitz, JE;Kuroda, MJ;Reimann, KA
通讯作者：
Reimann, KA