T-Cell-Receptor Repertoire in Chronic Plaque-Stage Psoriasis Is Restricted and Lacks Enrichment of Superantigen-Associated Vβ Regions
T-Cell-Receptor Repertoire in Chronic Plaque-Stage Psoriasis Is Restricted and Lacks Enrichment of Superantigen-Associated Vβ Regions
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慢性斑块期银屑病中的 T 细胞受体库受到限制并且缺乏超抗原相关 Vβ 区域的富集
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
W. Sterry
中科院分区：
文献类型：
--
作者：
W. Boehncke;D. Dressel;B. Manfras;T. Zollner;A. Wettstein;B. Böhm;W. Sterry
银屑病中淋巴细胞浸润对某些t细胞受体的优先使用可能表明抗原参与了这种疾病的发病机制。然而，到目前为止，还没有关于银屑病患者完整的t细胞受体Vα和Vβ库的数据。因此，我们通过半定量聚合酶链反应比较了10例慢性斑块期银屑病患者血液和皮肤中t细胞受体可变区域的使用情况。此外，通过序列特异性寡核苷酸分型分析HLA II类等位基因。在皮肤中观察到t细胞受体库的相当大的限制，其中高达20%的血液中存在的可变区域无法检测到。α-链和β-链都是如此。然而，没有推断出t细胞受体相互限制的恒定模式，不一致的是，某些β链在皮肤隔室内发生了一定的优先使用。这篇关于银屑病中完整的T细胞受体Vα和Vβ库的报道表明浸润T细胞的受体库受到限制，并且缺乏与超级抗原相关的Vβ区域的富集，因此超级抗原似乎在慢性斑块期银屑病中不起病理相关作用。
Preferential usage of certain T-cell receptors by the lymphocytic infiltrate in psoriasis might indicate the involvement of an antigen in the pathogenesis of this disease. However, to date there are no data on the complete T-cell-receptor Vα and Vβ repertoire in psoriatic patients. We therefore compared the usage of T-cell-receptor variable regions in blood and skin of 10 patients with chronic plaque-stage psoriasis by means of semiquantitative polymerase chain reaction. Additionally, HLA class II alleles were analyzed by means of sequence-specific oligonucleotide typing. A considerable restriction of the T.-cell-receptor repertoire was observed in the skin, where up to 20% of the variable regions present in the blood were not detectable. This was true for both α- and β-chains. However, no interindividually constant pattern of T-cell-receptor restriction was deducible, Inconsistently, a certain preferential usage of some β chains occurred within the cutaneous compartment. This report on the complete T-cell–receptor Vα and Vβ repertoire in psoriasis documents the restricted receptor repertoire of infiltrating T cells and a lack of enrichment of superantigen-associated Vβ regions, Thus superantigens seem not to play a pathogenetically relevant role in chronic plaque-stage psoriasis.
影响因子：
29.7
作者：
L. Picker;E. Butcher
通讯作者：
L. Picker;E. Butcher
UM4D4 (CDw60) T 细胞被划分到银屑病皮肤中并释放诱导银屑病皮损中表达的角质形成细胞表型的淋巴因子。
DOI：
10.1111/1523-1747.ep12484908
发表时间：
1990
期刊：
The Journal of investigative dermatology
影响因子：
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作者：
Baadsgaard,O;Tong,P;Elder,JT;Hansen,ER;Ho,V;Hammerberg,C;Lange-Vejlsgaard,G;Fox,DA;Fisher,G;Chan,LS
通讯作者：
Chan,LS
基于 PCR 的 T 细胞受体 V β 多基因家族分析：影响其有效性的实验参数。
影响因子：
2.7
作者：
Hall,BL;Finn,OJ
通讯作者：
Finn,OJ