A soil-binding polysaccharide complex released from root hairs functions in rhizosheath formation
A soil-binding polysaccharide complex released from root hairs functions in rhizosheath formation
复制标题
从根毛释放的土壤结合多糖复合物在根鞘形成中发挥作用
DOI：
10.1101/2021.04.15.440065
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Galloway A
中科院分区：
文献类型：
--
作者：
Galloway A
为了阐明与根鞘形成有关的因素，以野生型(WT)大麦为材料。CV。Pallas)和根无毛突变体秃根大麦(BRB)，采用生理生化和免疫化学相结合的方法进行了研究。在土壤中生长时，WT大麦根系与∼结合的土壤比单位根长多5倍。高相对分子质量(HMW)的菜籽多糖分泌物对土壤的结合力比根分泌物小。高分子量多糖渗出物的碳水化合物和葡聚糖单抗分析显示不同的糖链图谱。与WT植物相比，BRB的根分泌物降低了阿拉伯半乳糖蛋白(AGP)、延伸素和杂木聚糖表位的信号。相反，与WT相比，Brbroot分泌物含有的∼可检测到的木聚糖表位是WT的25倍。表位检测层析表明，木葡聚糖渗出物的检测增加是由于中性聚合物的丰度增加。Brb的渗出液制剂减少了与根毛定位的糖蛋白和杂木聚糖表位相关的酸性形式的木葡聚糖的数量，并与土壤结合特性有关。因此，除了形成土壤颗粒的物理结构外，根毛还通过释放与土壤结合的多糖复合体来促进根鞘的形成。一句话概述大麦根无毛突变体的根分泌物与野生型相比，具有改变的多糖表位模式和较少的酸性土壤结合多糖复合体。
To elucidate factors involved in rhizosheath formation, wild type (WT) barley (Hordeum vulgareL. cv. Pallas) and a root hairless mutant,bald root barley(brb), were investigated with a combination of physiological, biochemical and immunochemical assays. When grown in soil, WT barley roots bound ∼5-fold more soil thanbrbper unit root length. High molecular weight (HMW) polysaccharide exudates ofbrbroots had less soil-binding capacity than those of WT root exudates. Carbohydrate and glycan monoclonal antibody analyses of HMW polysaccharide exudates indicated differing glycan profiles. Relative to WT plants, root exudates ofbrbhad reduced signals for arabinogalactan-protein (AGP), extensin and heteroxylan epitopes thanbrb. In contrast, thebrbroot exudate contained ∼25-fold more detectable xyloglucan epitope relative to WT. Epitope detection chromatography indicated that the increased detection of xyloglucan inbrbexudates was due to enhanced abundance of a neutral polymer. Exudate preparations frombrbhad decreased amounts of an acidic form of xyloglucan associated with root-hair located glycoprotein and heteroxylan epitopes and with soil-binding properties. Therefore, in addition to physically structuring soil particles, root hairs facilitate rhizosheath formation by releasing a soil-binding polysaccharide complex.One sentence summaryThe root exudate of a root hairless mutant of barley, relative to wild type, has an altered pattern of polysaccharide epitopes and lesser amounts of an acidic soil-binding polysaccharide complex.
影响因子：
4.2
作者：
Holz, Maire;Zarebanadkouki, Mohsen;Carminati, Andrea
通讯作者：
Carminati, Andrea
影响因子：
5.6
作者：
Eldridge BM;Larson ER;Weldon L;Smyth KM;Sellin AN;Chenchiah IV;Liverpool TB;Grierson CS
通讯作者：
Grierson CS