Dynamic Reorganization of Nucleosome Positioning in Somatic Cells after Transfer into Porcine Enucleated Oocytes.
Dynamic Reorganization of Nucleosome Positioning in Somatic Cells after Transfer into Porcine Enucleated Oocytes.
复制标题
转移至猪去核卵母细胞后体细胞核小体定位的动态重组
DOI：
10.1016/j.stemcr.2017.06.004
复制
发表时间：
2017-08-08
影响因子：
5.9
通讯作者：
Miao YL
中科院分区：
文献类型：
--
作者：
Tao C;Li J;Zhang X;Chen B;Chi D;Zeng Y;Niu Y;Wang C;Cheng W;Wu W;Pan Z;Lian J;Liu H;Miao YL
核小体是染色质的基本结构单位，是基因表达的重要调节因子。体细胞重编程过程中核小体占据和定位的变化机制仍然知之甚少。我们建立了一种方法，用于生成猪胚胎成纤维细胞（PEF）的全基因组核小体图谱，通过体细胞核移植（SCNT）生成的重建1-细胞胚胎，以及使用MNase测序的受精卵（FZ），仅需1,000个细胞。结果发现，供体PEF基因导入猪卵母细胞后，染色体尤其是X染色体变得更加开放，核小体在启动子区的占有率降低，而在基因区的占有率增加。体细胞中不同表达水平的基因的转录起始位点周围的核小体排列在SCNT中趋于转录沉默;然而，一些多能性基因采用转录活性核小体排列。FZ和SCNT具有相似的特征，而不像PEF。这项研究揭示了核小体定位和染色质组织的动力学和重要性，在重编程后早期。在重编程中，核小体占据和分布发生了很大变化，多能基因的核小体在重编程中倾向于活跃排列，Liu，Miao及其同事探索了SCNT后猪成纤维细胞重编程期间的染色体动力学。通过应用低细胞输入的MNase-seq，这对稀缺的生物样品特别重要，他们揭示了核小体在重编程过程中的动态重组。
The nucleosome, the fundamental structural unit of chromatin, is a critical regulator of gene expression. The mechanisms governing changes to nucleosome occupancy and positioning during somatic cell reprogramming remain poorly understood. We established a method for generating genome-wide nucleosome maps of porcine embryonic fibroblasts (PEF), reconstructed 1-cell embryos generated by somatic cell nuclear transfer (SCNT), and fertilized zygotes (FZ) using MNase sequencing with only 1,000 cells. We found that donor PEF chromatin, especially X chromosome, became more open after transfer into porcine oocytes and nucleosome occupancy decreased in promoters but increased in the genic regions. Nucleosome arrangements around transcriptional start sites of genes with different expression levels in somatic cells tended to become transcriptionally silent in SCNT; however, some pluripotency genes adopted transcriptionally active nucleosome arrangements. FZ and SCNT had similar characteristics, unlike PEF. This study reveals the dynamics and importance of nucleosome positioning and chromatin organization early after reprogramming. MNase-seq was successfully conducted with as few as 1,000 cells Nucleosome occupancy and distribution changed considerably in reprogramming Nucleosome of pluripotent genes tended into active arrangements in reprogramming Liu, Miao, and colleagues explore the chromosome dynamics during reprogramming of porcine fibroblasts after SCNT. By applying MNase-seq with low cell input, which is particularly important for scarce biological samples, they reveal a dynamic reorganization of nucleosomes during reprogramming.
登录
查看更多内容
影响因子：
16.6
作者：
Cantone I;Bagci H;Dormann D;Dharmalingam G;Nesterova T;Brockdorff N;Rougeulle C;Vallot C;Heard E;Chaligne R;Merkenschlager M;Fisher AG
通讯作者：
Fisher AG
影响因子：
3.6
作者：
Vajta, G;Lewis, IM;Callesen, H
通讯作者：
Callesen, H
影响因子：
23.9
作者：
Johannesson, Bjarki;Sagi, Ido;Egli, Dieter
通讯作者：
Egli, Dieter
影响因子：
1.8
作者：
Kurome, Mayuko;Ishikawa, Takayuki;Nagashima, Hiroshi
通讯作者：
Nagashima, Hiroshi
影响因子：
1.7
作者：
Li, Juan;Li, Rong;Callesen, Andhenrik
通讯作者：
Callesen, Andhenrik