Genome-wide and molecular evolution analysis of the subtilase gene family in Vitis vinifera.
Genome-wide and molecular evolution analysis of the subtilase gene family in Vitis vinifera.
复制标题
葡萄枯草杆菌酶基因家族的全基因组和分子进化分析。
DOI：
10.1186/1471-2164-15-1116
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发表时间：
2014-12-16
期刊：
影响因子：
4.4
通讯作者：
Hu X
中科院分区：
文献类型：
--
作者：
Cao J;Han X;Zhang T;Yang Y;Huang J;Hu X
葡萄(Vitis Vinifera)是世界上最具经济意义的果树之一。最近公布的葡萄基因组序列的获得为鉴定和分析该物种中的一些重要基因家族提供了机会。枯草杆菌酶是一组与枯草杆菌蛋白类似的丝氨酸蛋白酶，参与植物体内的多种生物过程。然而，到目前为止，还没有关于葡萄的系统发育、染色体定位和基因复制、基因组织、功能分化、选择压力和表达谱等方面的全面研究报道。本研究对葡萄枯草酶基因家族进行了全面的分析。共鉴定出80个枯草杆菌酶基因。系统发育分析表明，这些枯草杆菌酶基因包括8个类群。基因组织在群体中相当保守。枯草杆菌酶基因在染色体上的分布是非随机的。这些基因中有很大一部分是优先聚集的，这表明串联复制可能对枯草杆菌酶基因家族的扩大做出了重大贡献。分歧和适应性进化的分析表明，虽然纯化选择可能是推动葡萄枯草杆菌酶进化的主要力量，但导致分歧的一些关键位点可能处于正选择之下。对实时PCR数据的进一步分析表明，许多枯草杆菌酶基因可能在植物的逆境反应和功能发育中发挥重要作用。串联复制以及纯化和正选择是导致葡萄枯草杆菌枯草杆菌酶基因功能差异的原因。这些数据可能有助于更好地理解葡萄枯草杆菌酶基因家族。本文的在线版本(DOI：10.1186/1471-2164-15-1116)包含补充材料，授权用户可以使用。
Vitis vinifera (grape) is one of the most economically significant fruit crops in the world. The availability of the recently released grape genome sequence offers an opportunity to identify and analyze some important gene families in this species. Subtilases are a group of subtilisin-like serine proteases that are involved in many biological processes in plants. However, no comprehensive study incorporating phylogeny, chromosomal location and gene duplication, gene organization, functional divergence, selective pressure and expression profiling has been reported so far for the grape. In the present study, a comprehensive analysis of the subtilase gene family in V. vinifera was performed. Eighty subtilase genes were identified. Phylogenetic analyses indicated that these subtilase genes comprised eight groups. The gene organization is considerably conserved among the groups. Distribution of the subtilase genes is non-random across the chromosomes. A high proportion of these genes are preferentially clustered, indicating that tandem duplications may have contributed significantly to the expansion of the subtilase gene family. Analyses of divergence and adaptive evolution show that while purifying selection may have been the main force driving the evolution of grape subtilases, some of the critical sites responsible for the divergence may have been under positive selection. Further analyses of real-time PCR data suggested that many subtilase genes might be important in the stress response and functional development of plants. Tandem duplications as well as purifying and positive selections have contributed to the functional divergence of subtilase genes in V. vinifera. The data may contribute to a better understanding of the grape subtilase gene family. The online version of this article (doi:10.1186/1471-2164-15-1116) contains supplementary material, which is available to authorized users.
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影响因子：
6.5
作者：
Budic, Maruska;Sabotic, Jerica;Kidric, Marjetka
通讯作者：
Kidric, Marjetka
影响因子：
11.4
作者：
Chen, Yuzhu;Hao, Xi;Cao, Jun
通讯作者：
Cao, Jun
DOI：
10.1073/pnas.162339999
发表时间：
2002-08-20
影响因子：
11.1
作者：
Gagne, JM;Downes, BP;Vierstra, RD
通讯作者：
Vierstra, RD
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
9.4
作者：
D'Erfurth, I.;Le Signor, C.;Gallardo, K.
通讯作者：
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