Developing a biohybrid lung - sufficient endothelialization of poly-4-methly-1-pentene gas exchange hollow-fiber membranes.
Developing a biohybrid lung - sufficient endothelialization of poly-4-methly-1-pentene gas exchange hollow-fiber membranes.
复制标题
开发生物混合肺——聚4-甲基-1-戊烯气体交换中空纤维膜的充分内皮化
DOI：
10.1016/j.jmbbm.2016.01.032
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发表时间：
2016
影响因子：
3.9
通讯作者：
Haverich A
中科院分区：
文献类型：
--
作者：
Wiegmann B;von Seggern H;Höffler K;Korossis S;Dipresa D;Pflaum M;Schmeckebier S;Seume J;Haverich A
为了建立具有最佳血液相容性的生物混合肺，本研究分析了在肝素/白蛋白涂层的聚-4-甲基-1-戊烯中空纤维气体交换膜（PMP-HFs）上建立流动阻力内皮的可行性。采用WST-8细胞增殖实验和荧光显微镜观察人脐血内皮细胞（HCBEC）在PMP-HFs上的接种效率和增殖情况。还评估了不同流动条件下的六氯代苯EC单层完整性。内皮特异性表型验证，表达激活水平和血栓形成状态标志物通过实时RT-PCR定量细胞与PMP-HF接触在静态和动态条件下。结果证明了在PMP-HF的血液接触表面上建立存活的、融合的和抗流动的内皮单层的可行性，其维持对TNFα刺激和流动条件的生理反应。内皮细胞表型、粘附分子表达水平和血栓形成状态标志物不受细胞与PMP-HFs接触的影响。这些结果代表了建立生物混合肺的重要一步。
Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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内皮细胞接种在假体表面。
DOI：
10.1615/jlongtermeffmedimplants.v10.i12.80
发表时间：
2000
期刊：
Journal of long-term effects of medical implants.
影响因子：
--
作者：
Consigny,PM
通讯作者：
Consigny,PM