Localisation and expression of TRPV6 in all intestinal segments and kidney of laying hens
Localisation and expression of TRPV6 in all intestinal segments and kidney of laying hens
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TRPV6在蛋鸡所有肠段和肾脏中的定位和表达
DOI：
10.1080/00071668.2011.596994
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发表时间：
2011-08
影响因子：
2
通讯作者：
Yu, Y.
中科院分区：
文献类型：
--
作者：
Yang, J. H.;Hou, J. F.;Farquharson, C.;Zhou, Z. L.;Deng, Y. F.;Wang, L.;Yu, Y.
1.本研究的目的是研究上皮钙通道TRPV6（transient receptor potential vanilloid channel type 6）在产蛋高峰期蛋鸡不同肠段和肾脏中的定位和表达。2.肠的免疫组织化学分析表明，TRPV6定位于十二指肠，空肠，回肠，盲肠和直肠的刷状缘膜。在直肠中表达较弱，在隐窝和杯状细胞中很少或没有表达。此外，TRPV6 mRNA在不同肠段中定量，并且在十二指肠和空肠中表达最高。Western blotting结果显示，TRPV6在十二指肠表达量最高，直肠表达量最低，其它肠段表达量居中。3.在肾脏中，在远曲小管（DCT）和髓连接小管（CNT）的顶端区域检测到TRPV 6的明显免疫阳性染色。有趣的是，TRPV6的分布延伸到近曲小管（PCT）。此外，肾脏表达较低的TRPV6 mRNA和蛋白水平相比，在十二指肠。4.总之，上皮钙通道TRPV6强烈表达在整个肠道和肾小管的顶端细胞，表明活性钙跨细胞转运在蛋鸡的膳食钙（再）吸收中起着至关重要的作用。
1. The aim of this study was to investigate the localisation and expression of the epithelial Ca2+ channel TRPV6 (transient receptor potential vanilloid channel type 6) in different intestinal segments and kidney of laying hens during peak lay. 2. Immunohistochemical analysis of the intestine indicated that TRPV6 was localised to the brush-border membranes of the duodenum, jejunum, ileum, caecum, and rectum. Expression was weaker in the rectum, and little or no expression was found in crypt and goblet cells. In addition, TRPV6 mRNA was quantified amongst different intestinal segments, and expression was highest in the duodenum and jejunum. Furthermore, Western blotting indicated that the duodenum expressed the greatest amount of TRPV6 and the rectum the least with the other segments expressing intermediate levels. 3. In the kidney, distinct immunopositive staining for TRPV6 was detected at the apical domain of the distal convoluted tubules (DCT) and medullary connecting tubules (CNT). Interestingly, distribution of TRPV6 extended to the proximal convoluted tubules (PCT). Furthermore, the kidney expressed lower TRPV6 mRNA and protein levels compared with that in the duodenum. 4. In conclusion, the epithelial Ca2+ channel TRPV6 is strongly expressed in the apical cells of the entire intestine and the renal tubules, suggesting that active Ca2+ transcellular transport plays a crucial role in dietary calcium (re)absorption in laying hens.
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影响因子：
2.2
作者：
M. Wilkens;C. Kunert-Keil;H. Brinkmeier;B. Schröder
通讯作者：
M. Wilkens;C. Kunert-Keil;H. Brinkmeier;B. Schröder
影响因子：
--
作者：
K. Ookata;A. Tojo;M. Onozato;M. Kashiwagi;S. Honda;S. Hirose
通讯作者：
K. Ookata;A. Tojo;M. Onozato;M. Kashiwagi;S. Honda;S. Hirose
影响因子：
3.1
作者：
K. Weber;Reinhold G. Erben;Andreas Rump;Jerzy Adamski
通讯作者：
K. Weber;Reinhold G. Erben;Andreas Rump;Jerzy Adamski
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
DOI：
10.1073/pnas.0505789102
发表时间：
2005-11-29
影响因子：
11.1
作者：
van der Eerden, BCJ;Hoenderop, JGJ;van Leeuwen, JPTM
通讯作者：
van Leeuwen, JPTM