A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking.
A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking.
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单次单位荧光探针，是单价，可光稳定且微量闪烁的。
作者：
单分子荧光技术已经成为理解复杂生物系统的一种强有力的方法。然而，研究人员仍然面临的一个挑战是几乎所有有机荧光团的光稳定性有限，包括花青和Alexa染料。我们报告了一种新的，单价探针，发射在远红外区的可见光谱的单分子光学成像所需的属性。该探针基于与聚甘油树枝状聚合物（PGD）缀合的环稠合硼-二吡咯亚甲基（BODIPY）核心。树枝状聚合物使疏水性荧光团水溶性。该探针具有优异的亮度，最大发射波长为705 nm。我们观察到，即使在不存在抗褪色剂如Trolox的情况下，单个PGD-BODIPY探针也具有惊人的长时间和稳定的发射，Trolox是一种常用于单分子研究以改善常见成像探针光稳定性的组合氧化还原剂。这些有趣的性质大大简化了荧光团的使用。
Single-molecule fluorescence techniques have emerged as a powerful approach to understand complex biological systems. However, a challenge researchers still face is the limited photostability of nearly all organic fluorophores, including the cyanine and Alexa dyes. We report a new, monovalent probe that emits in the far-red region of the visible spectrum with properties desirable for single-molecule optical imaging. This probe is based on a ring-fused boron-dipyrromethene (BODIPY) core that is conjugated to a polyglycerol dendrimer (PGD). The dendrimer makes the hydrophobic fluorophore water-soluble. This probe exhibits excellent brightness, with an emission maximum of 705 nm. We observed strikingly long and stable emission from individual PGD-BODIPY probes even in the absence of anti-fading agents such as Trolox, a combined oxidizing-reducing agent often used in single-molecule studies for improving the photostability of common imaging probes. These interesting properties greatly simplify use of the fluorophore.
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影响因子：
4.9
作者：
Atilgan, Serdar;Ekmekci, Zeynep;Akkaya, Engin U.
通讯作者：
Akkaya, Engin U.
影响因子：
4.9
作者：
Komatsu, Toru;Urano, Yasuteru;Nagano, Tetsuo
通讯作者：
Nagano, Tetsuo
影响因子：
62.1
作者：
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通讯作者：
Urano, Yasuteru
影响因子：
15
作者：
Cordes, Thorben;Vogelsang, Jan;Tinnefeld, Philip
通讯作者：
Tinnefeld, Philip
影响因子：
3.4
作者：
Dave, Richa;Terry, Daniel S.;Blanchard, Scott C.
通讯作者：
Blanchard, Scott C.