A comparison of chloroplast genome sequences in Aconitum (Ranunculaceae): a traditional herbal medicinal genus.
A comparison of chloroplast genome sequences in Aconitum (Ranunculaceae): a traditional herbal medicinal genus.
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传统草药属乌头（毛茛科）叶绿体基因组序列的比较
作者：
Kong H;Liu W;Yao G;Gong W
草本药用属蒲公英，属于毛茛科，代表真双子叶植物中最早的分化谱系。目前该属由两个亚属组成，A. Lycoctonum亚属和A. subg.好吧对三种植物的叶绿体基因组全序列进行了分析。angustius，A. finetianum和A.亚群中的中华蒙托菌并与其它豆科植物进行了比较，以阐明它们的系统发育关系，为豆科植物特别是东亚豆科植物的利用提供分子信息。叶绿体基因组序列长度为156，109 bp。angustius，155，625 bp; finetianum和A. sinomontanum，每个种有126个基因，其中蛋白质编码基因（PCG）84个。虽然基因组重排不存在，但在LSC/IR/SSC边界中检测到结构变异。鉴定了5个假基因，其中，LSC/IR/SSC边界中有19个假基因和15个假基因，LSC区域中有16个假基因和15个假基因，IRb区域中有15个假基因。核苷酸变异性（Pi）的估计为0.00549，与高变异的LSC和SSC比IR区域。8个基因间区域是高度可变的，共检测到58-62个简单重复序列（SSR）在所有三个物种。超过80%的SSR存在于LSC区域。在亚群中，单核苷酸类SSR分别占64.41%和46.81%。Lycoctonum和subg.而较高比例的二，三，四，五SSR存在于亚g。好吧大多数的subg。亚洲东部的古生物首先被用于系统发育分析。这些物种的cp基因组全序列的可用性亚g。Lycoctonum将有利于未来的系统发育分析，并有助于种质资源的利用。
The herbal medicinal genus Aconitum L., belonging to the Ranunculaceae family, represents the earliest diverging lineage within the eudicots. It currently comprises of two subgenera, A. subgenus Lycoctonum and A. subg. Aconitum. The complete chloroplast (cp) genome sequences were characterized in three species: A. angustius, A. finetianum, and A. sinomontanum in subg. Lycoctonum and compared to other Aconitum species to clarify their phylogenetic relationship and provide molecular information for utilization of Aconitum species particularly in Eastern Asia. The length of the chloroplast genome sequences were 156,109 bp in A. angustius, 155,625 bp in A. finetianum and 157,215 bp in A. sinomontanum, with each species possessing 126 genes with 84 protein coding genes (PCGs). While genomic rearrangements were absent, structural variation was detected in the LSC/IR/SSC boundaries. Five pseudogenes were identified, among which Ψrps19 and Ψycf1 were in the LSC/IR/SSC boundaries, Ψrps16 and ΨinfA in the LSC region, and Ψycf15 in the IRb region. The nucleotide variability (Pi) of Aconitum was estimated to be 0.00549, with comparably higher variations in the LSC and SSC than the IR regions. Eight intergenic regions were revealed to be highly variable and a total of 58–62 simple sequence repeats (SSRs) were detected in all three species. More than 80% of SSRs were present in the LSC region. Altogether, 64.41% and 46.81% of SSRs are mononucleotides in subg. Lycoctonum and subg. Aconitum, respectively, while a higher percentage of di-, tri-, tetra-, and penta- SSRs were present in subg. Aconitum. Most species of subg. Aconitum in Eastern Asia were first used for phylogenetic analyses. The availability of the complete cp genome sequences of these species in subg. Lycoctonum will benefit future phylogenetic analyses and aid in germplasm utilization in Aconitum species.
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影响因子：
4.1
作者：
Jabbour, Florian;Renner, Susanne S.
通讯作者：
Renner, Susanne S.
影响因子：
--
作者：
Lim, Chae Eun;Kim, Goon-Bo;Mun, Jeong-Hwan
通讯作者：
Mun, Jeong-Hwan
Aconitum barbatum var. 叶绿体基因组
影响因子：
5.6
作者：
Chen X;Li Q;Li Y;Qian J;Han J
通讯作者：
Han J
DOI：
10.1093/dnares/dsx006
发表时间：
2017-08-01
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Keller J;Rousseau-Gueutin M;Martin GE;Morice J;Boutte J;Coissac E;Ourari M;Aïnouche M;Salmon A;Cabello-Hurtado F;Aïnouche A
通讯作者：
Aïnouche A
影响因子：
4.1
作者：
Kim, KJ;Lee, HL
通讯作者：
Lee, HL