Chloroplast genome of Aconitum barbatum var. puberulum (Ranunculaceae) derived from CCS reads using the PacBio RS platform.
Chloroplast genome of Aconitum barbatum var. puberulum (Ranunculaceae) derived from CCS reads using the PacBio RS platform.
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Aconitum barbatum var. 叶绿体基因组
DOI：
10.3389/fpls.2015.00042
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发表时间：
2015
影响因子：
5.6
通讯作者：
Han J
中科院分区：
文献类型：
--
作者：
Chen X;Li Q;Li Y;Qian J;Han J
利用基于单分子实时（SMRT）测序方法的第三代测序平台对毛果铁棒锤（Aconitum barbatum var. puberulum）的叶绿体基因组（cp基因组）进行了测序。据我们所知，这是首次报道的乌头属完整叶绿体基因组，我们预计它将对毛茛科的系统发育研究具有重要价值。总共产生了23498条环形一致序列（CCS）读数和20685462个碱基对，平均读长为880bp，最长读长为2261bp。实现了100%的基因组覆盖度，平均覆盖度为132×且无间隙。组装基因组的准确性为99.973%；通过对叶绿体基因组中6个选定基因进行桑格测序对组装结果进行了验证。毛果铁棒锤的完整叶绿体基因组长度为156749bp，包括一个87630bp的大单拷贝区和一个16941bp的小单拷贝区，二者被两个26089bp的反向重复序列隔开。该叶绿体基因组包含130个基因，其中包括84个蛋白质编码基因、34个tRNA基因和8个rRNA基因。鉴定出4个正向重复、5个反向重复和8个串联重复。根据简单重复序列（SSR）分析，最长的多聚结构是一个20 - T重复。我们在本文中呈现的结果将有助于乌头属的系统发育研究和分子鉴定。
The chloroplast genome (cp genome) of Aconitum barbatum var. puberulum was sequenced using the third-generation sequencing platform based on the single-molecule real-time (SMRT) sequencing approach. To our knowledge, this is the first reported complete cp genome of Aconitum, and we anticipate that it will have great value for phylogenetic studies of the Ranunculaceae family. In total, 23,498 CCS reads and 20,685,462 base pairs were generated, the mean read length was 880 bp, and the longest read was 2,261 bp. Genome coverage of 100% was achieved with a mean coverage of 132× and no gaps. The accuracy of the assembled genome is 99.973%; the assembly was validated using Sanger sequencing of six selected genes from the cp genome. The complete cp genome of A. barbatum var. puberulum is 156,749 bp in length, including a large single-copy region of 87,630 bp and a small single-copy region of 16,941 bp separated by two inverted repeats of 26,089 bp. The cp genome contains 130 genes, including 84 protein-coding genes, 34 tRNA genes and eight rRNA genes. Four forward, five inverted and eight tandem repeats were identified. According to the SSR analysis, the longest poly structure is a 20-T repeat. Our results presented in this paper will facilitate the phylogenetic studies and molecular authentication on Aconitum.
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DOI：
10.1007/bf02464880
发表时间：
1989-06-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HIRATSUKA, J;SHIMADA, H;SUGIURA, M
通讯作者：
SUGIURA, M
影响因子：
10
作者：
Li, Xiwen;Yang, Yang;Chen, Shilin
通讯作者：
Chen, Shilin
影响因子：
5.4
作者：
Huang, JC;Sun, M
通讯作者：
Sun, M
影响因子：
2.5
作者：
Lohse, Marc;Drechsel, Oliver;Bock, Ralph
通讯作者：
Bock, Ralph
影响因子：
4.1
作者：
Jabbour, Florian;Renner, Susanne S.
通讯作者：
Renner, Susanne S.