Genomic evidence for the emergence and evolution of pathogenicity and niche preferences in the genus Campylobacter.
Genomic evidence for the emergence and evolution of pathogenicity and niche preferences in the genus Campylobacter.
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DOI：
10.1093/gbe/evu195
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发表时间：
2014-09-04
影响因子：
3.3
通讯作者：
Morsella C
中科院分区：
文献类型：
--
作者：
Iraola G;Pérez R;Naya H;Paolicchi F;Pastor E;Valenzuela S;Calleros L;Velilla A;Hernández M;Morsella C
弯曲杆菌属包括一些与人类和动物健康最相关的病原体;在其表征方面的持续努力也揭示了参与不同类型感染的新物种pupulmonary。如今，该属的可用基因组数据包括各种各样的物种，具有不同的致病潜力和生态位偏好。在这项工作中，我们有助于扩大这一可用的信息，提出了第一个基因组的物种弯曲杆菌BV。并使用这个和已经测序的生物体来分析弯曲杆菌属物种之间的致病性和生态位偏好的出现和演变。我们发现，弯曲杆菌可以明确区分建立和推定的病原体取决于他们的剧目的毒力基因，这已经从其他细菌水平收购，因为非致病性弯曲杆菌的祖先出现，和postorly互换的属的一些成员之间。此外，我们证明了水平基因转移和多样化进化在生态位偏好中的作用，能够区分与口腔，生殖器和胃肠道组织向性相关的遗传特征。特别是，我们强调了二硫键蛋白，入侵抗原B（Cia B），和其他分泌蛋白在确定生态位偏好的非同义进化的作用。我们的研究结果来自评估以前未实现的目标，即考虑整个可用的弯曲杆菌多样性进行基因组比较，揭示臭名昭著的遗传特征，这些遗传特征可以解释特定的表型，并为弯曲杆菌生物学的未来研究奠定基础。
The genus Campylobacter includes some of the most relevant pathogens for human and animal health; the continuous effort in their characterization has also revealed new species putatively involved in different kind of infections. Nowadays, the available genomic data for the genus comprise a wide variety of species with different pathogenic potential and niche preferences. In this work, we contribute to enlarge this available information presenting the first genome for the species Campylobacter sputorum bv. sputorum and use this and the already sequenced organisms to analyze the emergence and evolution of pathogenicity and niche preferences among Campylobacter species. We found that campylobacters can be unequivocally distinguished in established and putative pathogens depending on their repertory of virulence genes, which have been horizontally acquired from other bacteria because the nonpathogenic Campylobacter ancestor emerged, and posteriorly interchanged between some members of the genus. Additionally, we demonstrated the role of both horizontal gene transfers and diversifying evolution in niche preferences, being able to distinguish genetic features associated to the tropism for oral, genital, and gastrointestinal tissues. In particular, we highlight the role of nonsynonymous evolution of disulphide bond proteins, the invasion antigen B (CiaB), and other secreted proteins in the determination of niche preferences. Our results arise from assessing the previously unmet goal of considering the whole available Campylobacter diversity for genome comparisons, unveiling notorious genetic features that could explain particular phenotypes and set the basis for future research in Campylobacter biology.
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影响因子：
3.1
作者：
Grogono-Thomas, R;Dworkin, J;Newell, DG
通讯作者：
Newell, DG
影响因子：
3.1
作者：
Hagan, Erin C.;Mobley, Harry L. T.
通讯作者：
Mobley, Harry L. T.
DOI：
10.1073/pnas.0913033107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Jun, Se-Ran;Sims, Gregory E.;Kim, Sung-Hou
通讯作者：
Kim, Sung-Hou
影响因子：
3.1
作者：
KONKEL, ME;JOENS, LA
通讯作者：
JOENS, LA
影响因子：
1.9
作者：
Kara, D;Luppens, SBI;ten Cate, JM
通讯作者：
ten Cate, JM