Conserved mRNA-binding proteomes in eukaryotic organisms.
Conserved mRNA-binding proteomes in eukaryotic organisms.
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DOI：
10.1038/nsmb.3128
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发表时间：
2015-12
影响因子：
16.8
通讯作者：
Gerber AP
中科院分区：
文献类型：
--
作者：
Matia-González AM;Laing EE;Gerber AP
RNA结合蛋白（RBP）是基因表达的转录后调控所必需的。最近的高通量筛选已经大大增加了实验鉴定的RBP的数量;然而，全面鉴定生物体内的RBP是难以捉摸的。在这里，我们描述了765和594蛋白质的剧目，可重复地与多聚腺苷酸化的mRNA在酿酒酵母和秀丽隐杆线虫，分别相互作用。此外，我们报告的差异关联的mRBP细胞凋亡诱导后，在C。线虫L4期幼虫。引人注目的是，大多数蛋白质组成的mRNA结合蛋白质组（mRBPomes）是进化保守的酵母和C。包括早期代谢途径和蛋白酶体的组成部分。基于我们的证据表明，糖酵解酶结合到不同的糖酵解mRNA，我们推测，酶-mRNA相互作用涉及到一个古老的机制，转录后协调代谢途径，可能建立在从早期的RNA过渡到蛋白质的“世界”。
RNA-binding proteins (RBPs) are essential for the post-transcriptional regulation of gene expression. Recent high-throughput screens have dramatically increased the number of experimentally identified RBPs; however, comprehensive identification of RBPs within living organisms is elusive. Here we describe the repertoire of 765 and 594 proteins that reproducibly interact with polyadenylated mRNAs in Saccharomyces cerevisiae and Caenorhabditis elegans, respectively. Furthermore, we report the differential association of mRBPs upon apoptosis induction in C. elegans L4 stage larvae. Strikingly, most proteins comprising mRNA-binding proteomes (mRBPomes) are evolutionarily conserved between yeast and C. elegans, including components of early metabolic pathways and the proteasome. Based on our evidence that glycolytic enzymes bind to distinct glycolytic mRNAs, we speculate that enzyme-mRNA interactions relate to an ancient mechanism for post-transcriptional coordination of metabolic pathways, perhaps established during the transition from the early RNA to the protein ‘world’.
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影响因子：
3.7
作者：
Huang CY;Chen JY;Wu SC;Tan CH;Tzeng RY;Lu PJ;Wu YF;Chen RH;Wu YC
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Wu YC
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Hengartner, MO
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14.9
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64.8
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通讯作者：
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