Characterisation of SARS-CoV-2 Lentiviral Pseudotypes and Correlation between Pseudotype-Based Neutralisation Assays and Live Virus-Based Micro Neutralisation Assays.
Characterisation of SARS-CoV-2 Lentiviral Pseudotypes and Correlation between Pseudotype-Based Neutralisation Assays and Live Virus-Based Micro Neutralisation Assays.
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DOI：
10.3390/v12091011
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发表时间：
2020-09-10
期刊：
影响因子：
--
通讯作者：
Montomoli E
中科院分区：
文献类型：
--
作者：
Hyseni I;Molesti E;Benincasa L;Piu P;Casa E;Temperton NJ;Manenti A;Montomoli E
最近爆发的新型冠状病毒（SARS-CoV-2）及其在各大洲的迅速传播，迫切需要通过检测方法来检测人类血清或人类单克隆抗体针对 SARS-CoV-2 刺突蛋白的中和活性，并评估人类的血清学免疫能力。由于 SARS-CoV-2 活病毒微中和 (MN) 检测的可及性有限，并且需要加强生物防护，因此基于“假分型”的方法可以被认为是对其他血清学检测的有用补充。在充分表征带有 SARS-CoV-2 刺突蛋白的慢病毒假型后，我们将其用于基于假型的中和测定，以分析意大利血清流行病学研究中人血清样本的中和活性。通过基于假型的中和测定获得的结果反映了当针对野生型病毒测试同一组血清时获得的结果，显示基于假型的中和和MN结果的明显趋同。总体结果得出的结论是，基于假型的中和测定是使用野生型菌株的有效替代方法，尽管该系统需要优化和标准化，但它不仅可以补充经典血清学方法，而且还可以在无法采用其他方法时进行血清学评估，特别是在人类大流行背景下。
The recent outbreak of a novel Coronavirus (SARS-CoV-2) and its rapid spread across the continents has generated an urgent need for assays to detect the neutralising activity of human sera or human monoclonal antibodies against SARS-CoV-2 spike protein and to evaluate the serological immunity in humans. Since the accessibility of live virus microneutralisation (MN) assays with SARS-CoV-2 is limited and requires enhanced bio-containment, the approach based on “pseudotyping” can be considered a useful complement to other serological assays. After fully characterising lentiviral pseudotypes bearing the SARS-CoV-2 spike protein, we employed them in pseudotype-based neutralisation assays in order to profile the neutralising activity of human serum samples from an Italian sero-epidemiological study. The results obtained with pseudotype-based neutralisation assays mirrored those obtained when the same panel of sera was tested against the wild type virus, showing an evident convergence of the pseudotype-based neutralisation and MN results. The overall results lead to the conclusion that the pseudotype-based neutralisation assay is a valid alternative to using the wild-type strain, and although this system needs to be optimised and standardised, it can not only complement the classical serological methods, but also allows serological assessments to be made when other methods cannot be employed, especially in a human pandemic context.
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影响因子：
3.5
作者：
Geraerts M;Willems S;Baekelandt V;Debyser Z;Gijsbers R
通讯作者：
Gijsbers R
影响因子：
5.5
作者：
Logan N;McMonagle E;Drew AA;Takahashi E;McDonald M;Baron MD;Gilbert M;Cleaveland S;Haydon DT;Hosie MJ;Willett BJ
通讯作者：
Willett BJ
影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
影响因子：
1.2
作者：
Millet, Jean K.;Tang, Tiffany;Whittaker, Gary R.
通讯作者：
Whittaker, Gary R.
影响因子：
11.4
作者：
Li, WH;Zhang, CS;Sui, JH;Kuhn, JH;Moore, MJ;Luo, SW;Wong, SK;Huang, IC;Xu, KM;Vasilieva, N;Murakami, A;He, YQ;Marasco, WA;Guan, Y;Choe, HY;Farzan, M
通讯作者：
Farzan, M